Microgel-Assisted Delivery of Adenosine to Accelerate Fracture Healing.

Journal Article (Journal Article)

Extracellular adenosine plays a key role in promoting bone tissue formation. Local delivery of adenosine could be an effective therapeutic strategy to harness the beneficial effect of extracellular adenosine on bone tissue formation following injury. Herein, we describe the development of an injectable in situ curing scaffold containing microgel-based adenosine delivery units. The two-component scaffold includes adenosine-loaded microgels and functionalized hyaluronic acid (HA) molecules. The microgels were generated upon copolymerization of 3-acrylamidophenylboronic acid (3-APBA)- and 2-aminoethylmethacrylamide (2-AEMA)-conjugated HA (HA-AEMA) in an emulsion suspension. The PBA functional groups were used to load the adenosine molecules. Mixing of the microgels with the HA polymers containing clickable groups, dibenzocyclooctyne (DBCO) and azide (HA-DBCO and HA-Azide), resulted in a 3D scaffold embedded with adenosine delivery units. Application of the in situ curing scaffolds containing adenosine-loaded microgels following tibial fracture injury showed improved bone tissue healing in a mouse model as demonstrated by the reduced callus size, higher bone volume, and increased tissue mineral density compared to those treated with the scaffold without adenosine. Overall, our results suggest that local delivery of adenosine could potentially be an effective strategy to promote bone tissue repair.

Full Text

Duke Authors

Cited Authors

  • Hoque, J; Zeng, Y; Newman, H; Gonzales, G; Lee, C; Varghese, S

Published Date

  • November 14, 2022

Published In

Volume / Issue

  • 8 / 11

Start / End Page

  • 4863 - 4872

PubMed ID

  • 36266245

Electronic International Standard Serial Number (EISSN)

  • 2373-9878

Digital Object Identifier (DOI)

  • 10.1021/acsbiomaterials.2c00977


  • eng

Conference Location

  • United States