Hard top soft bottom microfluidic devices for cell culture and chemical analysis.

Journal Article (Journal Article)

We report fabrication and characterization of microfluidic devices made of thermoplastic and elastomeric polymers. These hard-soft hybrid material devices are motivated by the combined need for large scale manufacturability, enhanced barrier properties to gas permeation and evaporation of aqueous solutions compared to poly(dimethyl siloxane) (PDMS) devices, and compatibility with deformation-based actuation. Channel features are created on rigid polymers such as polyethylene terephthalate glycol (PETG), cyclic olefin copolymer (COC), and polystyrene (PS) by hot embossing. These "hard tops" are bonded to elastomeric "soft bottoms" (polyurethane (PU) or PDMS-parylene C-PDMS) to create devices that can be used for microfluidic cell culture where deformation-based fluid actuation schemes are used to perfuse and recirculate media. The higher barrier properties of this device compared to PDMS devices enable cell culture with less evaporation and creation of hypoxic conditions.

Full Text

Duke Authors

Cited Authors

  • Mehta, G; Lee, J; Cha, W; Tung, Y-C; Linderman, JJ; Takayama, S

Published Date

  • May 15, 2009

Published In

Volume / Issue

  • 81 / 10

Start / End Page

  • 3714 - 3722

PubMed ID

  • 19382754

Electronic International Standard Serial Number (EISSN)

  • 1520-6882

Digital Object Identifier (DOI)

  • 10.1021/ac802178u


  • eng

Conference Location

  • United States