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EMT Molecular Signatures of Pancreatic Neuroendocrine Neoplasms.

Publication ,  Journal Article
Venugopal, A; Michalczyk, A; Khasraw, M; Ackland, ML
Published in: Int J Mol Sci
November 7, 2022

Neuroendocrine neoplasms (NENs) are relatively rare neoplasms occurring predominantly in the gastrointestinal tract and pancreas. Their heterogeneity poses challenges for diagnosis and treatment. There is a paucity of markers for characterisation of NEN tumours. For routine diagnosis, immunohistochemistry of the NEN-specific markers CgA and synaptophysin and the proliferation marker Ki-67 are used. These parameters, however, are qualitative and lack the capacity to fully define the tumour phenotype. Molecules of epithelial-mesenchymal transition (EMT) are potential candidates for improved tumour characterisation. Using qRT-PCR, we measured mRNA levels of 27 tumour markers, including 25 EMT-associated markers, in tumour tissue and matched non-tumour tissues for 13 patients with pancreatic NENs. Tissue from patients with three different grades of tumour had distinctly different mRNA profiles. Of the 25 EMT-associated markers analysed, 17 were higher in G3 tissue relative to matched non-tumour tissue, including CD14, CD24, CD31, CD44, CD45, CD56, CK6, CK7, CK13, CK20, NSE, CDX2, CgA, DAXX, PCNA, laminin and Ki-67. The differences in levels of seven EMT-associated markers, Ki-67, DAXX, CD24, CD44, vimentin, laminin and PDX1 plus CgA and NSE (neuroendocrine markers) enabled a distinct molecular signature for each tumour grade to be generated. EMT molecules differentially expressed in three tumour grades have potential for use in tumour stratification and prognostication and as therapeutic targets for treatment of neuroendocrine cancers, following validation with additional samples.

Duke Scholars

Published In

Int J Mol Sci

DOI

EISSN

1422-0067

Publication Date

November 7, 2022

Volume

23

Issue

21

Location

Switzerland

Related Subject Headings

  • RNA, Messenger
  • Pancreatic Neoplasms
  • Laminin
  • Ki-67 Antigen
  • Humans
  • Epithelial-Mesenchymal Transition
  • Chemical Physics
  • Biomarkers, Tumor
  • 3404 Medicinal and biomolecular chemistry
  • 3107 Microbiology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Venugopal, A., Michalczyk, A., Khasraw, M., & Ackland, M. L. (2022). EMT Molecular Signatures of Pancreatic Neuroendocrine Neoplasms. Int J Mol Sci, 23(21). https://doi.org/10.3390/ijms232113645
Venugopal, Abhirami, Agnes Michalczyk, Mustafa Khasraw, and M Leigh Ackland. “EMT Molecular Signatures of Pancreatic Neuroendocrine Neoplasms.Int J Mol Sci 23, no. 21 (November 7, 2022). https://doi.org/10.3390/ijms232113645.
Venugopal A, Michalczyk A, Khasraw M, Ackland ML. EMT Molecular Signatures of Pancreatic Neuroendocrine Neoplasms. Int J Mol Sci. 2022 Nov 7;23(21).
Venugopal, Abhirami, et al. “EMT Molecular Signatures of Pancreatic Neuroendocrine Neoplasms.Int J Mol Sci, vol. 23, no. 21, Nov. 2022. Pubmed, doi:10.3390/ijms232113645.
Venugopal A, Michalczyk A, Khasraw M, Ackland ML. EMT Molecular Signatures of Pancreatic Neuroendocrine Neoplasms. Int J Mol Sci. 2022 Nov 7;23(21).

Published In

Int J Mol Sci

DOI

EISSN

1422-0067

Publication Date

November 7, 2022

Volume

23

Issue

21

Location

Switzerland

Related Subject Headings

  • RNA, Messenger
  • Pancreatic Neoplasms
  • Laminin
  • Ki-67 Antigen
  • Humans
  • Epithelial-Mesenchymal Transition
  • Chemical Physics
  • Biomarkers, Tumor
  • 3404 Medicinal and biomolecular chemistry
  • 3107 Microbiology