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The Pseudomonas syringae pv. syringae 61 hrpH product, an envelope protein required for elicitation of the hypersensitive response in plants.

Publication ,  Journal Article
Huang, HC; He, SY; Bauer, DW; Collmer, A
Published in: Journal of bacteriology
November 1992

Pseudomonas syringae pv. syringae 61 contains a 25-kb cluster of hrp genes that are required for elicitation of the hypersensitive response (HR) in tobacco. TnphoA mutagenesis of cosmid pHIR11, which contains the hrp cluster, revealed two genes encoding exported or inner-membrane-spanning proteins (H.-C. Huang, S. W. Hutcheson, and A. Collmer, Mol. Plant-Microbe Interact. 4:469-476, 1991). The gene in complementation group X, designated hrpH, was subcloned on a 3.1-kb SalI fragment into pCPP30, a broad-host-range, mobilizable vector. The subclone restored the ability of hrpH mutant P. syringae pv. syringae 61-2089 to elicit the HR in tobacco. DNA sequence analysis of the 3.1-kb SalI fragment revealed a single open reading frame encoding an 81,956-Da preprotein with a typical amino-terminal signal peptide and no likely inner-membrane-spanning hydrophobic regions. hrpH was expressed in the presence of [35S]methionine by using the T7 RNA polymerase-promoter system and vector pT7-3 in Escherichia coli and was shown to encode a protein with an apparent molecular weight of 83,000 on sodium dodecyl sulfate-polyacrylamide gels. The HrpH protein in E. coli was located in the membrane fraction and was absent from the periplasm and cytoplasm. The HrpH protein possessed similarity with several outer membrane proteins that are known to be involved in protein or phage secretion, including the Klebsiella oxytoca PulD protein, the Yersinia enterocolitica YscC protein, and the pIV protein of filamentous coliphages. All of these proteins possess a possible secretion motif, GG(X)12VP(L/F)LXXIPXIGXL(F/L), near the carboxyl terminus, and they lack a carboxyl-terminal phenylalanine, in contrast to other outer membrane proteins with no known secretion function. These results suggest that the P. syringae pv. syringae HrpH protein is involved in the secretion of a proteinaceous HR elicitor.

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Published In

Journal of bacteriology

DOI

EISSN

1098-5530

ISSN

0021-9193

Publication Date

November 1992

Volume

174

Issue

21

Start / End Page

6878 / 6885

Related Subject Headings

  • Suppression, Genetic
  • Subcellular Fractions
  • Sequence Homology, Amino Acid
  • Pseudomonas
  • Plants
  • Plant Physiological Phenomena
  • Phenotype
  • Molecular Sequence Data
  • Microbiology
  • Membrane Proteins
 

Citation

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Huang, H. C., He, S. Y., Bauer, D. W., & Collmer, A. (1992). The Pseudomonas syringae pv. syringae 61 hrpH product, an envelope protein required for elicitation of the hypersensitive response in plants. Journal of Bacteriology, 174(21), 6878–6885. https://doi.org/10.1128/jb.174.21.6878-6885.1992
Huang, H. C., S. Y. He, D. W. Bauer, and A. Collmer. “The Pseudomonas syringae pv. syringae 61 hrpH product, an envelope protein required for elicitation of the hypersensitive response in plants.Journal of Bacteriology 174, no. 21 (November 1992): 6878–85. https://doi.org/10.1128/jb.174.21.6878-6885.1992.
Huang, H. C., et al. “The Pseudomonas syringae pv. syringae 61 hrpH product, an envelope protein required for elicitation of the hypersensitive response in plants.Journal of Bacteriology, vol. 174, no. 21, Nov. 1992, pp. 6878–85. Epmc, doi:10.1128/jb.174.21.6878-6885.1992.

Published In

Journal of bacteriology

DOI

EISSN

1098-5530

ISSN

0021-9193

Publication Date

November 1992

Volume

174

Issue

21

Start / End Page

6878 / 6885

Related Subject Headings

  • Suppression, Genetic
  • Subcellular Fractions
  • Sequence Homology, Amino Acid
  • Pseudomonas
  • Plants
  • Plant Physiological Phenomena
  • Phenotype
  • Molecular Sequence Data
  • Microbiology
  • Membrane Proteins