Skip to main content
Journal cover image

Pretargeting and Bioorthogonal Click Chemistry-Mediated Endogenous Stem Cell Homing for Heart Repair.

Publication ,  Journal Article
Li, Z; Shen, D; Hu, S; Su, T; Huang, K; Liu, F; Hou, L; Cheng, K
Published in: ACS Nano
December 26, 2018

Stem cell therapy is one of the promising strategies for the treatment of ischemic heart disease. However, the clinical application of stem cells transplantation is limited by low cell engraftment in the infarcted myocardium. Taking advantage of pretargeting and bioorthogonal chemistry, we engineered a pretargeting and bioorthogonal chemistry (PTBC) system to capture endogenous circulating stem cells and target them to the injured heart for effective repair. Two bioorthogonal antibodies were i.v. administrated with a pretargeting interval (48 h). Through bioorthogonal click reaction, the two antibodies are linked in vivo, engaging endogenous stem cells with circulating platelets. As a result, the platelets redirect the stem cells to the injured heart. In vitro and in vivo studies demonstrated that bioorthogonal click reaction was able to induce the conjugation of platelets and endothelial progenitor cells (EPCs) and enhance the binding of EPCs to collagen and injured blood vessels. More importantly, in a mouse model of acute myocardial infarction, the in vivo results of cardiac function, heart morphometry, and immunohistochemistry assessment all confirmed effective heart repair by the PTBC system.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

ACS Nano

DOI

EISSN

1936-086X

Publication Date

December 26, 2018

Volume

12

Issue

12

Start / End Page

12193 / 12200

Location

United States

Related Subject Headings

  • Stem Cells
  • Stem Cell Transplantation
  • Nanoscience & Nanotechnology
  • Molecular Structure
  • Mice, Inbred C57BL
  • Mice
  • Humans
  • Heart Diseases
  • Endothelial Progenitor Cells
  • Disease Models, Animal
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Li, Z., Shen, D., Hu, S., Su, T., Huang, K., Liu, F., … Cheng, K. (2018). Pretargeting and Bioorthogonal Click Chemistry-Mediated Endogenous Stem Cell Homing for Heart Repair. ACS Nano, 12(12), 12193–12200. https://doi.org/10.1021/acsnano.8b05892
Li, Zhenhua, Deliang Shen, Shiqi Hu, Teng Su, Ke Huang, Feiran Liu, Lei Hou, and Ke Cheng. “Pretargeting and Bioorthogonal Click Chemistry-Mediated Endogenous Stem Cell Homing for Heart Repair.ACS Nano 12, no. 12 (December 26, 2018): 12193–200. https://doi.org/10.1021/acsnano.8b05892.
Li Z, Shen D, Hu S, Su T, Huang K, Liu F, et al. Pretargeting and Bioorthogonal Click Chemistry-Mediated Endogenous Stem Cell Homing for Heart Repair. ACS Nano. 2018 Dec 26;12(12):12193–200.
Li, Zhenhua, et al. “Pretargeting and Bioorthogonal Click Chemistry-Mediated Endogenous Stem Cell Homing for Heart Repair.ACS Nano, vol. 12, no. 12, Dec. 2018, pp. 12193–200. Pubmed, doi:10.1021/acsnano.8b05892.
Li Z, Shen D, Hu S, Su T, Huang K, Liu F, Hou L, Cheng K. Pretargeting and Bioorthogonal Click Chemistry-Mediated Endogenous Stem Cell Homing for Heart Repair. ACS Nano. 2018 Dec 26;12(12):12193–12200.
Journal cover image

Published In

ACS Nano

DOI

EISSN

1936-086X

Publication Date

December 26, 2018

Volume

12

Issue

12

Start / End Page

12193 / 12200

Location

United States

Related Subject Headings

  • Stem Cells
  • Stem Cell Transplantation
  • Nanoscience & Nanotechnology
  • Molecular Structure
  • Mice, Inbred C57BL
  • Mice
  • Humans
  • Heart Diseases
  • Endothelial Progenitor Cells
  • Disease Models, Animal