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Expression of endocrine gland-derived vascular endothelial growth factor in ovarian carcinoma.

Publication ,  Journal Article
Zhang, L; Yang, N; Conejo-Garcia, J-R; Katsaros, D; Mohamed-Hadley, A; Fracchioli, S; Schlienger, K; Toll, A; Levine, B; Rubin, SC; Coukos, G
Published in: Clin Cancer Res
January 2003

The first tissue-specific angiogenic molecule, endocrine gland-derived vascular endothelial growth factor (EG-VEGF), was identified recently in human ovary, raising hopes of developing tumor type-specific angiogenesis inhibitors. In the present study, we analyzed the expression of EG-VEGF mRNA in normal human tissues and ovarian neoplasms by quantitative real-time reverse transcription-PCR. EG-VEGF mRNA was expressed in all ovarian neoplasms examined. No significant difference was identified among benign, low malignant potential neoplasms or stage I ovarian cancer, all of which exhibited 2-fold lower mRNA levels compared with normal premenopausal ovaries. EG-VEGF mRNA levels further decreased in late stage compared with early stage carcinomas (P < 0.05) and were consistently lower in laser capture microdissected tumor islets compared with surrounding stroma. EG-VEGF was undetectable by reverse transcription-PCR in 17 established epithelial ovarian cancer cell lines or in cultured human ovarian surface epithelial cells, whereas it was detected in peripheral blood as well as tumor-infiltrating T lymphocytes. Finally, in contrast to VEGF, EG-VEGF mRNA levels did not correlate with clinical outcome in advanced ovarian carcinoma. These results suggest that EG-VEGF is most likely derived from nonepithelial components of ovarian carcinomas and may play a marginal role in promoting angiogenesis in advanced ovarian carcinoma. We postulate that EG-VEGF-targeted antiangiogenic therapy may prove useful in early stage but not in advanced stage ovarian carcinoma.

Duke Scholars

Published In

Clin Cancer Res

ISSN

1078-0432

Publication Date

January 2003

Volume

9

Issue

1

Start / End Page

264 / 272

Location

United States

Related Subject Headings

  • Vascular Endothelial Growth Factors
  • Vascular Endothelial Growth Factor, Endocrine-Gland-Derived
  • Vascular Endothelial Growth Factor A
  • Tumor Cells, Cultured
  • Treatment Outcome
  • Tissue Distribution
  • Time Factors
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Ovarian Neoplasms
 

Citation

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Zhang, L., Yang, N., Conejo-Garcia, J.-R., Katsaros, D., Mohamed-Hadley, A., Fracchioli, S., … Coukos, G. (2003). Expression of endocrine gland-derived vascular endothelial growth factor in ovarian carcinoma. Clin Cancer Res, 9(1), 264–272.
Zhang, Lin, Nuo Yang, Jose-Ramon Conejo-Garcia, Dionyssios Katsaros, Alisha Mohamed-Hadley, Stefano Fracchioli, Katia Schlienger, et al. “Expression of endocrine gland-derived vascular endothelial growth factor in ovarian carcinoma.Clin Cancer Res 9, no. 1 (January 2003): 264–72.
Zhang L, Yang N, Conejo-Garcia J-R, Katsaros D, Mohamed-Hadley A, Fracchioli S, et al. Expression of endocrine gland-derived vascular endothelial growth factor in ovarian carcinoma. Clin Cancer Res. 2003 Jan;9(1):264–72.
Zhang, Lin, et al. “Expression of endocrine gland-derived vascular endothelial growth factor in ovarian carcinoma.Clin Cancer Res, vol. 9, no. 1, Jan. 2003, pp. 264–72.
Zhang L, Yang N, Conejo-Garcia J-R, Katsaros D, Mohamed-Hadley A, Fracchioli S, Schlienger K, Toll A, Levine B, Rubin SC, Coukos G. Expression of endocrine gland-derived vascular endothelial growth factor in ovarian carcinoma. Clin Cancer Res. 2003 Jan;9(1):264–272.

Published In

Clin Cancer Res

ISSN

1078-0432

Publication Date

January 2003

Volume

9

Issue

1

Start / End Page

264 / 272

Location

United States

Related Subject Headings

  • Vascular Endothelial Growth Factors
  • Vascular Endothelial Growth Factor, Endocrine-Gland-Derived
  • Vascular Endothelial Growth Factor A
  • Tumor Cells, Cultured
  • Treatment Outcome
  • Tissue Distribution
  • Time Factors
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Ovarian Neoplasms