Expression of endocrine gland-derived vascular endothelial growth factor in ovarian carcinoma.

Journal Article (Journal Article)

The first tissue-specific angiogenic molecule, endocrine gland-derived vascular endothelial growth factor (EG-VEGF), was identified recently in human ovary, raising hopes of developing tumor type-specific angiogenesis inhibitors. In the present study, we analyzed the expression of EG-VEGF mRNA in normal human tissues and ovarian neoplasms by quantitative real-time reverse transcription-PCR. EG-VEGF mRNA was expressed in all ovarian neoplasms examined. No significant difference was identified among benign, low malignant potential neoplasms or stage I ovarian cancer, all of which exhibited 2-fold lower mRNA levels compared with normal premenopausal ovaries. EG-VEGF mRNA levels further decreased in late stage compared with early stage carcinomas (P < 0.05) and were consistently lower in laser capture microdissected tumor islets compared with surrounding stroma. EG-VEGF was undetectable by reverse transcription-PCR in 17 established epithelial ovarian cancer cell lines or in cultured human ovarian surface epithelial cells, whereas it was detected in peripheral blood as well as tumor-infiltrating T lymphocytes. Finally, in contrast to VEGF, EG-VEGF mRNA levels did not correlate with clinical outcome in advanced ovarian carcinoma. These results suggest that EG-VEGF is most likely derived from nonepithelial components of ovarian carcinomas and may play a marginal role in promoting angiogenesis in advanced ovarian carcinoma. We postulate that EG-VEGF-targeted antiangiogenic therapy may prove useful in early stage but not in advanced stage ovarian carcinoma.

Full Text

Duke Authors

Cited Authors

  • Zhang, L; Yang, N; Conejo-Garcia, J-R; Katsaros, D; Mohamed-Hadley, A; Fracchioli, S; Schlienger, K; Toll, A; Levine, B; Rubin, SC; Coukos, G

Published Date

  • January 2003

Published In

Volume / Issue

  • 9 / 1

Start / End Page

  • 264 - 272

PubMed ID

  • 12538479

International Standard Serial Number (ISSN)

  • 1078-0432


  • eng

Conference Location

  • United States