A Decade of CRISPR-Cas Gnome Editing in C. elegans .

Journal Article (Review;Journal Article)

CRISPR-Cas allows us to introduce desired genome editing, including mutations, epitopes, and deletions, with unprecedented efficiency. The development of CRISPR-Cas has progressed to such an extent that it is now applicable in various fields, with the help of model organisms. C. elegans is one of the pioneering animals in which numerous CRISPR-Cas strategies have been rapidly established over the past decade. Ironically, the emergence of numerous methods makes the choice of the correct method difficult. Choosing an appropriate selection or screening approach is the first step in planning a genome modification. This report summarizes the key features and applications of CRISPR-Cas methods using C. elegans , illustrating key strategies. Our overview of significant advances in CRISPR-Cas will help readers understand the current advances in genome editing and navigate various methods of CRISPR-Cas genome editing.

Full Text

Duke Authors

Cited Authors

  • Kim, H-M; Hong, Y; Chen, J

Published Date

  • December 2022

Published In

Volume / Issue

  • 23 / 24

Start / End Page

  • 15863 -

PubMed ID

  • 36555505

Pubmed Central ID

  • PMC9781986

Electronic International Standard Serial Number (EISSN)

  • 1422-0067

International Standard Serial Number (ISSN)

  • 1422-0067

Digital Object Identifier (DOI)

  • 10.3390/ijms232415863


  • eng