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Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E.

Publication ,  Journal Article
Nal, B; Chan, C; Kien, F; Siu, L; Tse, J; Chu, K; Kam, J; Staropoli, I; Crescenzo-Chaigne, B; Escriou, N; van der Werf, S; Yuen, K-Y; Altmeyer, R
Published in: The Journal of general virology
May 2005

Post-translational modifications and correct subcellular localization of viral structural proteins are prerequisites for assembly and budding of enveloped viruses. Coronaviruses, like the severe acute respiratory syndrome-associated virus (SARS-CoV), bud from the endoplasmic reticulum-Golgi intermediate compartment. In this study, the subcellular distribution and maturation of SARS-CoV surface proteins S, M and E were analysed by using C-terminally tagged proteins. As early as 30 min post-entry into the endoplasmic reticulum, high-mannosylated S assembles into trimers prior to acquisition of complex N-glycans in the Golgi. Like S, M acquires high-mannose N-glycans that are subsequently modified into complex N-glycans in the Golgi. The N-glycosylation profile and the absence of O-glycosylation on M protein relate SARS-CoV to the previously described group 1 and 3 coronaviruses. Immunofluorescence analysis shows that S is detected in several compartments along the secretory pathway from the endoplasmic reticulum to the plasma membrane while M predominantly localizes in the Golgi, where it accumulates, and in trafficking vesicles. The E protein is not glycosylated. Pulse-chase labelling and confocal microscopy in the presence of protein translation inhibitor cycloheximide revealed that the E protein has a short half-life of 30 min. E protein is found in bright perinuclear patches colocalizing with endoplasmic reticulum markers. In conclusion, SARS-CoV surface proteins S, M and E show differential subcellular localizations when expressed alone suggesting that additional cellular or viral factors might be required for coordinated trafficking to the virus assembly site in the endoplasmic reticulum-Golgi intermediate compartment.

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Published In

The Journal of general virology

DOI

EISSN

1465-2099

ISSN

0022-1317

Publication Date

May 2005

Volume

86

Issue

Pt 5

Start / End Page

1423 / 1434

Related Subject Headings

  • Virology
  • Viral Matrix Proteins
  • Viral Envelope Proteins
  • Spike Glycoprotein, Coronavirus
  • Severe acute respiratory syndrome-related coronavirus
  • Protein Transport
  • Protein Processing, Post-Translational
  • Polysaccharides
  • Microscopy, Confocal
  • Membrane Glycoproteins
 

Citation

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Nal, B., Chan, C., Kien, F., Siu, L., Tse, J., Chu, K., … Altmeyer, R. (2005). Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E. The Journal of General Virology, 86(Pt 5), 1423–1434. https://doi.org/10.1099/vir.0.80671-0
Nal, Béatrice, Cheman Chan, Francois Kien, Lewis Siu, Jane Tse, Kid Chu, Jason Kam, et al. “Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E.The Journal of General Virology 86, no. Pt 5 (May 2005): 1423–34. https://doi.org/10.1099/vir.0.80671-0.
Nal B, Chan C, Kien F, Siu L, Tse J, Chu K, et al. Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E. The Journal of general virology. 2005 May;86(Pt 5):1423–34.
Nal, Béatrice, et al. “Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E.The Journal of General Virology, vol. 86, no. Pt 5, May 2005, pp. 1423–34. Epmc, doi:10.1099/vir.0.80671-0.
Nal B, Chan C, Kien F, Siu L, Tse J, Chu K, Kam J, Staropoli I, Crescenzo-Chaigne B, Escriou N, van der Werf S, Yuen K-Y, Altmeyer R. Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E. The Journal of general virology. 2005 May;86(Pt 5):1423–1434.

Published In

The Journal of general virology

DOI

EISSN

1465-2099

ISSN

0022-1317

Publication Date

May 2005

Volume

86

Issue

Pt 5

Start / End Page

1423 / 1434

Related Subject Headings

  • Virology
  • Viral Matrix Proteins
  • Viral Envelope Proteins
  • Spike Glycoprotein, Coronavirus
  • Severe acute respiratory syndrome-related coronavirus
  • Protein Transport
  • Protein Processing, Post-Translational
  • Polysaccharides
  • Microscopy, Confocal
  • Membrane Glycoproteins