High performance liquid chromatography/electrospray ionization mass spectrometry for simultaneous analysis of alkamides and caffeic acid derivatives from Echinacea purpurea extracts.

Journal Article (Journal Article)

Extracts of the plant Echinacea purpurea are widely used for medicinal purposes. Effective quality control of these extracts requires rapid methods to determine their chemical composition. A new method for analysis of caffeic acid derivatives and alkamides from Echinacea extracts has been developed. With this method, isomeric isobutylamides and 2-methylbutylamides can be distinguished, a capability that previously published methods have lacked. Quantitative analyses carried out with this method on E. purpurea extracts that have been stored for 18 months indicate that they contain caftaric acid, cichoric acid, and undeca-2Z,4E-diene-8,10-diynoic acid isobutylamide at concentrations of 0.7, 0.71 and 2.0mg/mL, respectively.

Full Text

Duke Authors

Cited Authors

  • Cech, NB; Eleazer, MS; Shoffner, LT; Crosswhite, MR; Davis, AC; Mortenson, AM

Published Date

  • January 27, 2006

Published In

Volume / Issue

  • 1103 / 2

Start / End Page

  • 219 - 228

PubMed ID

  • 16298376

International Standard Serial Number (ISSN)

  • 0021-9673

Digital Object Identifier (DOI)

  • 10.1016/j.chroma.2005.11.008


  • eng

Conference Location

  • Netherlands