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COUP-TFII regulates human endometrial stromal genes involved in inflammation.

Publication ,  Journal Article
Li, X; Large, MJ; Creighton, CJ; Lanz, RB; Jeong, J-W; Young, SL; Lessey, BA; Palomino, WA; Tsai, SY; Demayo, FJ
Published in: Mol Endocrinol
December 2013

Chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII; NR2F2) is an orphan nuclear receptor involved in cell-fate specification, organogenesis, angiogenesis, and metabolism. Ablation of COUP-TFII in the mouse uterus causes infertility due to defects in embryo attachment and impaired uterine stromal cell decidualization. Although the function of COUP-TFII in uterine decidualization has been described in mice, its role in the human uterus remains unknown. We observed that, as in mice, COUP-TFII is robustly expressed in the endometrial stroma of healthy women, and its expression is reduced in the ectopic lesions of women with endometriosis. To interrogate the role of COUP-TFII in human endometrial function, we used a small interfering RNA-mediated loss of function approach in primary human endometrial stromal cells. Attenuation of COUP-TFII expression did not completely block decidualization; rather it had a selective effect on gene expression. To better elucidate the role of COUP-TFII in endometrial stroma cell biology, the COUP-TFII transcriptome was defined by pairing microarray comparison with chromatin immunoprecipitation followed by deep sequencing. Gene ontology analysis demonstrates that COUP-TFII regulates a subset of genes in endometrial stroma cell decidualization such as those involved in cell adhesion, angiogenesis, and inflammation. Importantly this analysis shows that COUP-TFII plays a role in controlling the expression of inflammatory cytokines. The determination that COUP-TFII plays a role in inflammation may add insight into the role of COUP-TFII in embryo implantation and in endometrial diseases such as endometriosis.

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Published In

Mol Endocrinol

DOI

EISSN

1944-9917

Publication Date

December 2013

Volume

27

Issue

12

Start / End Page

2041 / 2054

Location

United States

Related Subject Headings

  • Young Adult
  • Stromal Cells
  • Signal Transduction
  • Reproducibility of Results
  • RNA, Small Interfering
  • Protein Binding
  • Oligonucleotide Array Sequence Analysis
  • Middle Aged
  • Mice
  • Inflammation
 

Citation

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Li, X., Large, M. J., Creighton, C. J., Lanz, R. B., Jeong, J.-W., Young, S. L., … Demayo, F. J. (2013). COUP-TFII regulates human endometrial stromal genes involved in inflammation. Mol Endocrinol, 27(12), 2041–2054. https://doi.org/10.1210/me.2013-1191
Li, Xilong, Michael J. Large, Chad J. Creighton, Rainer B. Lanz, Jae-Wook Jeong, Steven L. Young, Bruce A. Lessey, Wilder A. Palomino, Sophia Y. Tsai, and Francesco J. Demayo. “COUP-TFII regulates human endometrial stromal genes involved in inflammation.Mol Endocrinol 27, no. 12 (December 2013): 2041–54. https://doi.org/10.1210/me.2013-1191.
Li X, Large MJ, Creighton CJ, Lanz RB, Jeong J-W, Young SL, et al. COUP-TFII regulates human endometrial stromal genes involved in inflammation. Mol Endocrinol. 2013 Dec;27(12):2041–54.
Li, Xilong, et al. “COUP-TFII regulates human endometrial stromal genes involved in inflammation.Mol Endocrinol, vol. 27, no. 12, Dec. 2013, pp. 2041–54. Pubmed, doi:10.1210/me.2013-1191.
Li X, Large MJ, Creighton CJ, Lanz RB, Jeong J-W, Young SL, Lessey BA, Palomino WA, Tsai SY, Demayo FJ. COUP-TFII regulates human endometrial stromal genes involved in inflammation. Mol Endocrinol. 2013 Dec;27(12):2041–2054.

Published In

Mol Endocrinol

DOI

EISSN

1944-9917

Publication Date

December 2013

Volume

27

Issue

12

Start / End Page

2041 / 2054

Location

United States

Related Subject Headings

  • Young Adult
  • Stromal Cells
  • Signal Transduction
  • Reproducibility of Results
  • RNA, Small Interfering
  • Protein Binding
  • Oligonucleotide Array Sequence Analysis
  • Middle Aged
  • Mice
  • Inflammation