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Determination of separate inhibitor and substrate binding sites in the dehaloperoxidase-hemoglobin from Amphitrite ornata.

Publication ,  Journal Article
Davis, MF; Bobay, BG; Franzen, S
Published in: Biochemistry
February 16, 2010

Dehaloperoxidase-hemoglobin (DHP A) is a dual function protein found in the terrebellid polychaete Amphitrite ornata. A. ornata is an annelid, which inhabits estuary mudflats with other polychaetes that secrete a range of toxic brominated phenols. DHP A is capable of binding and oxidatively dehalogenating some of these compounds. DHP A possesses the ability to bind halophenols in a distinct, internal distal binding pocket. Since its discovery, the distal binding pocket has been reported as the sole binding location for halophenols; however, data herein suggest a distinction between inhibitor (monohalogenated phenol) and substrate (trihalogenated phenol) binding locations. Backbone (13)Calpha, (13)Cbeta, carbonyl (13)C, amide (1)H, and amide (15)N resonance assignments have been made, and various halophenols were titrated into the protein. (1)H-(15)N HSQC experiments were collected at stoichiometric intervals during each titration, and binding locations specific for mono- and trihalogenated phenols have been identified. Titration of monohalogenated phenol induced primary changes around the distal binding pocket, while introduction of trihalogenated phenols created alterations of the distal histidine and the local area surrounding W120, a structural region that corresponds to a possible dimer interface region recently observed in X-ray crystal structures of DHP A.

Duke Scholars

Published In

Biochemistry

DOI

EISSN

1520-4995

Publication Date

February 16, 2010

Volume

49

Issue

6

Start / End Page

1199 / 1206

Location

United States

Related Subject Headings

  • Substrate Specificity
  • Spectrophotometry, Ultraviolet
  • Polychaeta
  • Phenols
  • Peroxidases
  • Magnetic Resonance Spectroscopy
  • Hydrocarbons, Halogenated
  • Hemoglobins
  • Chlorophenols
  • Catalytic Domain
 

Citation

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Davis, M. F., Bobay, B. G., & Franzen, S. (2010). Determination of separate inhibitor and substrate binding sites in the dehaloperoxidase-hemoglobin from Amphitrite ornata. Biochemistry, 49(6), 1199–1206. https://doi.org/10.1021/bi9018576
Davis, Michael F., Benjamin G. Bobay, and Stefan Franzen. “Determination of separate inhibitor and substrate binding sites in the dehaloperoxidase-hemoglobin from Amphitrite ornata.Biochemistry 49, no. 6 (February 16, 2010): 1199–1206. https://doi.org/10.1021/bi9018576.
Davis, Michael F., et al. “Determination of separate inhibitor and substrate binding sites in the dehaloperoxidase-hemoglobin from Amphitrite ornata.Biochemistry, vol. 49, no. 6, Feb. 2010, pp. 1199–206. Pubmed, doi:10.1021/bi9018576.
Journal cover image

Published In

Biochemistry

DOI

EISSN

1520-4995

Publication Date

February 16, 2010

Volume

49

Issue

6

Start / End Page

1199 / 1206

Location

United States

Related Subject Headings

  • Substrate Specificity
  • Spectrophotometry, Ultraviolet
  • Polychaeta
  • Phenols
  • Peroxidases
  • Magnetic Resonance Spectroscopy
  • Hydrocarbons, Halogenated
  • Hemoglobins
  • Chlorophenols
  • Catalytic Domain