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Insights into the nature of DNA binding of AbrB-like transcription factors.

Publication ,  Journal Article
Sullivan, DM; Bobay, BG; Kojetin, DJ; Thompson, RJ; Rance, M; Strauch, MA; Cavanagh, J
Published in: Structure
November 12, 2008

Understanding the DNA recognition and binding by the AbrB-like family of transcriptional regulators is of significant interest since these proteins enable bacteria to elicit the appropriate response to diverse environmental stimuli. Although these "transition-state regulator" proteins have been well characterized at the genetic level, the general and specific mechanisms of DNA binding remain elusive. We present RDC-refined NMR solution structures and dynamic properties of the DNA-binding domains of three Bacillus subtilis transition-state regulators: AbrB, Abh, and SpoVT. We combined previously investigated DNase I footprinting, DNA methylation, gel-shift assays, and mutagenic and NMR studies to generate a structural model of the complex between AbrBN(55) and its cognate promoter, abrB8. These investigations have enabled us to generate a model for the specific nature of the transition-state regulator-DNA interaction, a structure that has remained elusive thus far.

Duke Scholars

Published In

Structure

DOI

ISSN

0969-2126

Publication Date

November 12, 2008

Volume

16

Issue

11

Start / End Page

1702 / 1713

Location

United States

Related Subject Headings

  • Transcription Factors
  • Thermodynamics
  • Protein Conformation
  • Models, Molecular
  • Magnetic Resonance Spectroscopy
  • Hydrogen Bonding
  • DNA-Binding Proteins
  • DNA
  • Biophysics
  • Binding Sites
 

Citation

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ICMJE
MLA
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Sullivan, D. M., Bobay, B. G., Kojetin, D. J., Thompson, R. J., Rance, M., Strauch, M. A., & Cavanagh, J. (2008). Insights into the nature of DNA binding of AbrB-like transcription factors. Structure, 16(11), 1702–1713. https://doi.org/10.1016/j.str.2008.08.014
Sullivan, Daniel M., Benjamin G. Bobay, Douglas J. Kojetin, Richele J. Thompson, Mark Rance, Mark A. Strauch, and John Cavanagh. “Insights into the nature of DNA binding of AbrB-like transcription factors.Structure 16, no. 11 (November 12, 2008): 1702–13. https://doi.org/10.1016/j.str.2008.08.014.
Sullivan DM, Bobay BG, Kojetin DJ, Thompson RJ, Rance M, Strauch MA, et al. Insights into the nature of DNA binding of AbrB-like transcription factors. Structure. 2008 Nov 12;16(11):1702–13.
Sullivan, Daniel M., et al. “Insights into the nature of DNA binding of AbrB-like transcription factors.Structure, vol. 16, no. 11, Nov. 2008, pp. 1702–13. Pubmed, doi:10.1016/j.str.2008.08.014.
Sullivan DM, Bobay BG, Kojetin DJ, Thompson RJ, Rance M, Strauch MA, Cavanagh J. Insights into the nature of DNA binding of AbrB-like transcription factors. Structure. 2008 Nov 12;16(11):1702–1713.
Journal cover image

Published In

Structure

DOI

ISSN

0969-2126

Publication Date

November 12, 2008

Volume

16

Issue

11

Start / End Page

1702 / 1713

Location

United States

Related Subject Headings

  • Transcription Factors
  • Thermodynamics
  • Protein Conformation
  • Models, Molecular
  • Magnetic Resonance Spectroscopy
  • Hydrogen Bonding
  • DNA-Binding Proteins
  • DNA
  • Biophysics
  • Binding Sites