Design considerations for analyzing protein translation regulation by condensates.

Journal Article (Journal Article;Review)

One proposed role for biomolecular condensates that contain RNA is translation regulation. In several specific contexts, translation has been shown to be modulated by the presence of a phase-separating protein and under conditions which promote phase separation, and likely many more await discovery. A powerful tool for determining the rules for condensate-dependent translation is the use of engineered RNA sequences, which can serve as reporters for translation efficiency. This Perspective will discuss design features to consider in engineering RNA reporters to determine the role of phase separation in translational regulation. Specifically, we will cover (i) how to engineer RNA sequence to recapitulate native protein/RNA interactions, (ii) the advantages and disadvantages for commonly used reporter RNA sequences, and (iii) important control experiments to distinguish between binding- and condensation-dependent translational repression. The goal of this review is to promote the design and application of faithful translation reporters to demonstrate a physiological role of biomolecular condensates in translation.

Full Text

Duke Authors

Cited Authors

  • Roden, CA; Gladfelter, AS

Published Date

  • January 2022

Published In

Volume / Issue

  • 28 / 1

Start / End Page

  • 88 - 96

PubMed ID

  • 34670845

Pubmed Central ID

  • PMC8675288

Electronic International Standard Serial Number (EISSN)

  • 1469-9001

Digital Object Identifier (DOI)

  • 10.1261/rna.079002.121


  • eng

Conference Location

  • United States