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Interplay of septin amphipathic helices in sensing membrane-curvature and filament bundling.

Publication ,  Journal Article
Woods, BL; Cannon, KS; Vogt, EJD; Crutchley, JM; Gladfelter, AS
Published in: Mol Biol Cell
October 1, 2021

The curvature of the membrane defines cell shape. Septins are GTP-binding proteins that assemble into heteromeric complexes and polymerize into filaments at areas of micron-scale membrane curvature. An amphipathic helix (AH) domain within the septin complex is necessary and sufficient for septins to preferentially assemble onto micron-scale curvature. Here we report that the nonessential fungal septin, Shs1, also has an AH domain capable of recognizing membrane curvature. In a septin mutant strain lacking a fully functional Cdc12 AH domain (cdc12-6), the C-terminal extension of Shs1, containing an AH domain, becomes essential. Additionally, we find that the Cdc12 AH domain is important for regulating septin filament bundling, suggesting septin AH domains have multiple, distinct functions and that bundling and membrane binding may be coordinately controlled.

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Published In

Mol Biol Cell

DOI

EISSN

1939-4586

Publication Date

October 1, 2021

Volume

32

Issue

20

Start / End Page

br5

Location

United States

Related Subject Headings

  • Septins
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Protein Domains
  • Membrane Proteins
  • Developmental Biology
  • Cytoskeleton
  • Cytoskeletal Proteins
  • Cell Shape
  • Cell Cycle Proteins
 

Citation

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Woods, B. L., Cannon, K. S., Vogt, E. J. D., Crutchley, J. M., & Gladfelter, A. S. (2021). Interplay of septin amphipathic helices in sensing membrane-curvature and filament bundling. Mol Biol Cell, 32(20), br5. https://doi.org/10.1091/mbc.E20-05-0303
Woods, Benjamin L., Kevin S. Cannon, Ellysa J. D. Vogt, John M. Crutchley, and Amy S. Gladfelter. “Interplay of septin amphipathic helices in sensing membrane-curvature and filament bundling.Mol Biol Cell 32, no. 20 (October 1, 2021): br5. https://doi.org/10.1091/mbc.E20-05-0303.
Woods BL, Cannon KS, Vogt EJD, Crutchley JM, Gladfelter AS. Interplay of septin amphipathic helices in sensing membrane-curvature and filament bundling. Mol Biol Cell. 2021 Oct 1;32(20):br5.
Woods, Benjamin L., et al. “Interplay of septin amphipathic helices in sensing membrane-curvature and filament bundling.Mol Biol Cell, vol. 32, no. 20, Oct. 2021, p. br5. Pubmed, doi:10.1091/mbc.E20-05-0303.
Woods BL, Cannon KS, Vogt EJD, Crutchley JM, Gladfelter AS. Interplay of septin amphipathic helices in sensing membrane-curvature and filament bundling. Mol Biol Cell. 2021 Oct 1;32(20):br5.

Published In

Mol Biol Cell

DOI

EISSN

1939-4586

Publication Date

October 1, 2021

Volume

32

Issue

20

Start / End Page

br5

Location

United States

Related Subject Headings

  • Septins
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Protein Domains
  • Membrane Proteins
  • Developmental Biology
  • Cytoskeleton
  • Cytoskeletal Proteins
  • Cell Shape
  • Cell Cycle Proteins