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Phosphoregulation provides specificity to biomolecular condensates in the cell cycle and cell polarity.

Publication ,  Journal Article
Gerbich, TM; McLaughlin, GA; Cassidy, K; Gerber, S; Adalsteinsson, D; Gladfelter, AS
Published in: J Cell Biol
July 6, 2020

Biomolecular condensation is a way of organizing cytosol in which proteins and nucleic acids coassemble into compartments. In the multinucleate filamentous fungus Ashbya gossypii, the RNA-binding protein Whi3 regulates the cell cycle and cell polarity through forming macromolecular structures that behave like condensates. Whi3 has distinct spatial localizations and mRNA targets, making it a powerful model for how, when, and where specific identities are established for condensates. We identified residues on Whi3 that are differentially phosphorylated under specific conditions and generated mutants that ablate this regulation. This yielded separation of function alleles that were functional for either cell polarity or nuclear cycling but not both. This study shows that phosphorylation of individual residues on molecules in biomolecular condensates can provide specificity that gives rise to distinct functional identities in the same cell.

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Published In

J Cell Biol

DOI

EISSN

1540-8140

Publication Date

July 6, 2020

Volume

219

Issue

7

Location

United States

Related Subject Headings

  • Stress, Physiological
  • RNA-Binding Proteins
  • RNA, Messenger
  • RNA, Fungal
  • Protein Processing, Post-Translational
  • Phosphorylation
  • Mutation
  • Hot Temperature
  • Gene Expression
  • Fungal Proteins
 

Citation

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Gerbich, T. M., McLaughlin, G. A., Cassidy, K., Gerber, S., Adalsteinsson, D., & Gladfelter, A. S. (2020). Phosphoregulation provides specificity to biomolecular condensates in the cell cycle and cell polarity. J Cell Biol, 219(7). https://doi.org/10.1083/jcb.201910021
Gerbich, Therese M., Grace A. McLaughlin, Katelyn Cassidy, Scott Gerber, David Adalsteinsson, and Amy S. Gladfelter. “Phosphoregulation provides specificity to biomolecular condensates in the cell cycle and cell polarity.J Cell Biol 219, no. 7 (July 6, 2020). https://doi.org/10.1083/jcb.201910021.
Gerbich TM, McLaughlin GA, Cassidy K, Gerber S, Adalsteinsson D, Gladfelter AS. Phosphoregulation provides specificity to biomolecular condensates in the cell cycle and cell polarity. J Cell Biol. 2020 Jul 6;219(7).
Gerbich, Therese M., et al. “Phosphoregulation provides specificity to biomolecular condensates in the cell cycle and cell polarity.J Cell Biol, vol. 219, no. 7, July 2020. Pubmed, doi:10.1083/jcb.201910021.
Gerbich TM, McLaughlin GA, Cassidy K, Gerber S, Adalsteinsson D, Gladfelter AS. Phosphoregulation provides specificity to biomolecular condensates in the cell cycle and cell polarity. J Cell Biol. 2020 Jul 6;219(7).

Published In

J Cell Biol

DOI

EISSN

1540-8140

Publication Date

July 6, 2020

Volume

219

Issue

7

Location

United States

Related Subject Headings

  • Stress, Physiological
  • RNA-Binding Proteins
  • RNA, Messenger
  • RNA, Fungal
  • Protein Processing, Post-Translational
  • Phosphorylation
  • Mutation
  • Hot Temperature
  • Gene Expression
  • Fungal Proteins