Absolute Arrangement of Subunits in Cytoskeletal Septin Filaments in Cells Measured by Fluorescence Microscopy.
Publication
, Journal Article
Kaplan, C; Jing, B; Winterflood, CM; Bridges, AA; Occhipinti, P; Schmied, J; Grinhagens, S; Gronemeyer, T; Tinnefeld, P; Gladfelter, AS ...
Published in: Nano Lett
June 10, 2015
We resolved the organization of subunits in cytoskeletal polymers in cells by light microscopy. Septin GTPases form linear complexes of about 32 nm length that polymerize into filaments. We visualized both termini of septin complexes by single molecule microscopy in vitro. Complexes appeared as 32 nm spaced localization pairs, and filaments appeared as stretches of equidistant localizations. Cellular septins were resolved as localization pairs and thin stretches of equidistant localizations.
Duke Scholars
Altmetric Attention Stats
Dimensions Citation Stats
Published In
Nano Lett
DOI
EISSN
1530-6992
Publication Date
June 10, 2015
Volume
15
Issue
6
Start / End Page
3859 / 3864
Location
United States
Related Subject Headings
- Septins
- Saccharomyces cerevisiae Proteins
- Saccharomyces cerevisiae
- Nanoscience & Nanotechnology
- Microscopy, Fluorescence
- Cytoskeleton
Citation
APA
Chicago
ICMJE
MLA
NLM
Kaplan, C., Jing, B., Winterflood, C. M., Bridges, A. A., Occhipinti, P., Schmied, J., … Ewers, H. (2015). Absolute Arrangement of Subunits in Cytoskeletal Septin Filaments in Cells Measured by Fluorescence Microscopy. Nano Lett, 15(6), 3859–3864. https://doi.org/10.1021/acs.nanolett.5b00693
Kaplan, Charlotte, Bo Jing, Christian M. Winterflood, Andrew A. Bridges, Patricia Occhipinti, Jürgen Schmied, Sören Grinhagens, et al. “Absolute Arrangement of Subunits in Cytoskeletal Septin Filaments in Cells Measured by Fluorescence Microscopy.” Nano Lett 15, no. 6 (June 10, 2015): 3859–64. https://doi.org/10.1021/acs.nanolett.5b00693.
Kaplan C, Jing B, Winterflood CM, Bridges AA, Occhipinti P, Schmied J, et al. Absolute Arrangement of Subunits in Cytoskeletal Septin Filaments in Cells Measured by Fluorescence Microscopy. Nano Lett. 2015 Jun 10;15(6):3859–64.
Kaplan, Charlotte, et al. “Absolute Arrangement of Subunits in Cytoskeletal Septin Filaments in Cells Measured by Fluorescence Microscopy.” Nano Lett, vol. 15, no. 6, June 2015, pp. 3859–64. Pubmed, doi:10.1021/acs.nanolett.5b00693.
Kaplan C, Jing B, Winterflood CM, Bridges AA, Occhipinti P, Schmied J, Grinhagens S, Gronemeyer T, Tinnefeld P, Gladfelter AS, Ries J, Ewers H. Absolute Arrangement of Subunits in Cytoskeletal Septin Filaments in Cells Measured by Fluorescence Microscopy. Nano Lett. 2015 Jun 10;15(6):3859–3864.
Published In
Nano Lett
DOI
EISSN
1530-6992
Publication Date
June 10, 2015
Volume
15
Issue
6
Start / End Page
3859 / 3864
Location
United States
Related Subject Headings
- Septins
- Saccharomyces cerevisiae Proteins
- Saccharomyces cerevisiae
- Nanoscience & Nanotechnology
- Microscopy, Fluorescence
- Cytoskeleton