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Subcellular mRNA localization and local translation of Arhgap11a in radial glial progenitors regulates cortical development.

Publication ,  Journal Article
Pilaz, L-J; Liu, J; Joshi, K; Tsunekawa, Y; Musso, CM; D'Arcy, BR; Suzuki, IK; Alsina, FC; Kc, P; Sethi, S; Vanderhaeghen, P; Polleux, F; Silver, DL
Published in: Neuron
March 15, 2023

mRNA localization and local translation enable exquisite spatial and temporal control of gene expression, particularly in polarized, elongated cells. These features are especially prominent in radial glial cells (RGCs), which are neural and glial precursors of the developing cerebral cortex and scaffolds for migrating neurons. Yet the mechanisms by which subcellular RGC compartments accomplish their diverse functions are poorly understood. Here, we demonstrate that mRNA localization and local translation of the RhoGAP ARHGAP11A in the basal endfeet of RGCs control their morphology and mediate neuronal positioning. Arhgap11a transcript and protein exhibit conserved localization to RGC basal structures in mice and humans, conferred by the 5' UTR. Proper RGC morphology relies upon active Arhgap11a mRNA transport and localization to the basal endfeet, where ARHGAP11A is locally synthesized. This translation is essential for positioning interneurons at the basement membrane. Thus, local translation spatially and acutely activates Rho signaling in RGCs to compartmentalize neural progenitor functions.

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Published In

Neuron

DOI

EISSN

1097-4199

Publication Date

March 15, 2023

Volume

111

Issue

6

Start / End Page

839 / 856.e5

Location

United States

Related Subject Headings

  • RNA, Messenger
  • Neurology & Neurosurgery
  • Neuroglia
  • Neurogenesis
  • Mice
  • Humans
  • GTPase-Activating Proteins
  • Ependymoglial Cells
  • Cerebral Cortex
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
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Pilaz, L.-J., Liu, J., Joshi, K., Tsunekawa, Y., Musso, C. M., D’Arcy, B. R., … Silver, D. L. (2023). Subcellular mRNA localization and local translation of Arhgap11a in radial glial progenitors regulates cortical development. Neuron, 111(6), 839-856.e5. https://doi.org/10.1016/j.neuron.2023.02.023
Pilaz, Louis-Jan, Jing Liu, Kaumudi Joshi, Yuji Tsunekawa, Camila M. Musso, Brooke R. D’Arcy, Ikuo K. Suzuki, et al. “Subcellular mRNA localization and local translation of Arhgap11a in radial glial progenitors regulates cortical development.Neuron 111, no. 6 (March 15, 2023): 839-856.e5. https://doi.org/10.1016/j.neuron.2023.02.023.
Pilaz L-J, Liu J, Joshi K, Tsunekawa Y, Musso CM, D’Arcy BR, et al. Subcellular mRNA localization and local translation of Arhgap11a in radial glial progenitors regulates cortical development. Neuron. 2023 Mar 15;111(6):839-856.e5.
Pilaz, Louis-Jan, et al. “Subcellular mRNA localization and local translation of Arhgap11a in radial glial progenitors regulates cortical development.Neuron, vol. 111, no. 6, Mar. 2023, pp. 839-856.e5. Pubmed, doi:10.1016/j.neuron.2023.02.023.
Pilaz L-J, Liu J, Joshi K, Tsunekawa Y, Musso CM, D’Arcy BR, Suzuki IK, Alsina FC, Kc P, Sethi S, Vanderhaeghen P, Polleux F, Silver DL. Subcellular mRNA localization and local translation of Arhgap11a in radial glial progenitors regulates cortical development. Neuron. 2023 Mar 15;111(6):839-856.e5.
Journal cover image

Published In

Neuron

DOI

EISSN

1097-4199

Publication Date

March 15, 2023

Volume

111

Issue

6

Start / End Page

839 / 856.e5

Location

United States

Related Subject Headings

  • RNA, Messenger
  • Neurology & Neurosurgery
  • Neuroglia
  • Neurogenesis
  • Mice
  • Humans
  • GTPase-Activating Proteins
  • Ependymoglial Cells
  • Cerebral Cortex
  • Animals