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Basolateral secretions of human endometrial epithelial organoids impact stromal cell decidualization.

Publication ,  Journal Article
Fitzgerald, HC; Kelleher, AM; Ranjit, C; Schust, DJ; Spencer, TE
Published in: Molecular human reproduction
April 2023

Uterine glands and, by inference, their secretions impact uterine receptivity, blastocyst implantation, stromal cell decidualization, and placental development. Changes in gland function across the menstrual cycle are primarily governed by the steroid hormones estrogen (E2) and progesterone (P4) but can also be influenced by extrinsic factors from the stroma. Using a human endometrial epithelial organoid system, transcriptome and proteome analyses identified distinct responses of the organoids to steroid hormones and prostaglandin E2 (PGE2). Notably, P4 and PGE2 modulated the basolateral secretion of organoid proteins, particularly cystatin C (CST3), serpin family A member 3 (SERPINA3), and stanniocalcin 1 (STC1). CST3, but not SERPINA3 or STC1, attenuated the in vitro stromal decidualization response to steroid hormones and PGE2. These findings provide evidence that uterine gland-derived factors impact stromal cell decidualization, which has implications for pregnancy establishment and fertility in women.

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Published In

Molecular human reproduction

DOI

EISSN

1460-2407

ISSN

1360-9947

Publication Date

April 2023

Volume

29

Issue

4

Start / End Page

gaad007

Related Subject Headings

  • Stromal Cells
  • Progesterone
  • Pregnancy
  • Placenta
  • Obstetrics & Reproductive Medicine
  • Humans
  • Female
  • Endometrium
  • Embryo Implantation
  • Dinoprostone
 

Citation

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ICMJE
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Fitzgerald, H. C., Kelleher, A. M., Ranjit, C., Schust, D. J., & Spencer, T. E. (2023). Basolateral secretions of human endometrial epithelial organoids impact stromal cell decidualization. Molecular Human Reproduction, 29(4), gaad007. https://doi.org/10.1093/molehr/gaad007
Fitzgerald, Harriet C., Andrew M. Kelleher, Chaman Ranjit, Danny J. Schust, and Thomas E. Spencer. “Basolateral secretions of human endometrial epithelial organoids impact stromal cell decidualization.Molecular Human Reproduction 29, no. 4 (April 2023): gaad007. https://doi.org/10.1093/molehr/gaad007.
Fitzgerald HC, Kelleher AM, Ranjit C, Schust DJ, Spencer TE. Basolateral secretions of human endometrial epithelial organoids impact stromal cell decidualization. Molecular human reproduction. 2023 Apr;29(4):gaad007.
Fitzgerald, Harriet C., et al. “Basolateral secretions of human endometrial epithelial organoids impact stromal cell decidualization.Molecular Human Reproduction, vol. 29, no. 4, Apr. 2023, p. gaad007. Epmc, doi:10.1093/molehr/gaad007.
Fitzgerald HC, Kelleher AM, Ranjit C, Schust DJ, Spencer TE. Basolateral secretions of human endometrial epithelial organoids impact stromal cell decidualization. Molecular human reproduction. 2023 Apr;29(4):gaad007.
Journal cover image

Published In

Molecular human reproduction

DOI

EISSN

1460-2407

ISSN

1360-9947

Publication Date

April 2023

Volume

29

Issue

4

Start / End Page

gaad007

Related Subject Headings

  • Stromal Cells
  • Progesterone
  • Pregnancy
  • Placenta
  • Obstetrics & Reproductive Medicine
  • Humans
  • Female
  • Endometrium
  • Embryo Implantation
  • Dinoprostone