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Drug Target Elucidation Through Isolation and Analysis of Drug-Resistant Mutants in Cryptococcus neoformans.

Publication ,  Journal Article
Hoy, MJ; Heitman, J
Published in: Methods Mol Biol
2023

Drug target identification is an essential component to antifungal drug development. Many methods, including large chemical library screening, natural product screening, and drug repurposing efforts, can identify compounds with favorable in vitro antifungal activity. However, these approaches will often identify compounds with no known mechanism of action. Herein, we describe a method utilizing the human fungal pathogen Cryptococcus neoformans to identify antifungal drug targets through the isolation of spontaneous resistant mutants, antifungal testing, whole-genome sequencing, and variant analysis.

Duke Scholars

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2023

Volume

2658

Start / End Page

127 / 143

Location

United States

Related Subject Headings

  • Humans
  • Developmental Biology
  • Cryptococcus neoformans
  • Cryptococcosis
  • Antifungal Agents
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
  • 0399 Other Chemical Sciences
 

Citation

APA
Chicago
ICMJE
MLA
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Hoy, M. J., & Heitman, J. (2023). Drug Target Elucidation Through Isolation and Analysis of Drug-Resistant Mutants in Cryptococcus neoformans. Methods Mol Biol, 2658, 127–143. https://doi.org/10.1007/978-1-0716-3155-3_9
Hoy, Michael J., and Joseph Heitman. “Drug Target Elucidation Through Isolation and Analysis of Drug-Resistant Mutants in Cryptococcus neoformans.Methods Mol Biol 2658 (2023): 127–43. https://doi.org/10.1007/978-1-0716-3155-3_9.
Hoy, Michael J., and Joseph Heitman. “Drug Target Elucidation Through Isolation and Analysis of Drug-Resistant Mutants in Cryptococcus neoformans.Methods Mol Biol, vol. 2658, 2023, pp. 127–43. Pubmed, doi:10.1007/978-1-0716-3155-3_9.

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2023

Volume

2658

Start / End Page

127 / 143

Location

United States

Related Subject Headings

  • Humans
  • Developmental Biology
  • Cryptococcus neoformans
  • Cryptococcosis
  • Antifungal Agents
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
  • 0399 Other Chemical Sciences