Skip to main content

Large-scale interrogation of retinal cell functions by 1-photon light-sheet microscopy.

Publication ,  Journal Article
Roy, S; Wang, D; Rudzite, AM; Perry, B; Scalabrino, ML; Thapa, M; Gong, Y; Sher, A; Field, GD
Published in: Cell Rep Methods
April 24, 2023

Visual processing in the retina depends on the collective activity of large ensembles of neurons organized in different layers. Current techniques for measuring activity of layer-specific neural ensembles rely on expensive pulsed infrared lasers to drive 2-photon activation of calcium-dependent fluorescent reporters. We present a 1-photon light-sheet imaging system that can measure the activity in hundreds of neurons in the ex vivo retina over a large field of view while presenting visual stimuli. This allows for a reliable functional classification of different retinal cell types. We also demonstrate that the system has sufficient resolution to image calcium entry at individual synaptic release sites across the axon terminals of dozens of simultaneously imaged bipolar cells. The simple design, large field of view, and fast image acquisition make this a powerful system for high-throughput and high-resolution measurements of retinal processing at a fraction of the cost of alternative approaches.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Cell Rep Methods

DOI

EISSN

2667-2375

Publication Date

April 24, 2023

Volume

3

Issue

4

Start / End Page

100453

Location

United States

Related Subject Headings

  • Neurons
  • Microscopy
  • Law Enforcement
  • Coloring Agents
  • Calcium, Dietary
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Roy, S., Wang, D., Rudzite, A. M., Perry, B., Scalabrino, M. L., Thapa, M., … Field, G. D. (2023). Large-scale interrogation of retinal cell functions by 1-photon light-sheet microscopy. Cell Rep Methods, 3(4), 100453. https://doi.org/10.1016/j.crmeth.2023.100453
Roy, Suva, Depeng Wang, Andra M. Rudzite, Benjamin Perry, Miranda L. Scalabrino, Mishek Thapa, Yiyang Gong, Alexander Sher, and Greg D. Field. “Large-scale interrogation of retinal cell functions by 1-photon light-sheet microscopy.Cell Rep Methods 3, no. 4 (April 24, 2023): 100453. https://doi.org/10.1016/j.crmeth.2023.100453.
Roy S, Wang D, Rudzite AM, Perry B, Scalabrino ML, Thapa M, et al. Large-scale interrogation of retinal cell functions by 1-photon light-sheet microscopy. Cell Rep Methods. 2023 Apr 24;3(4):100453.
Roy, Suva, et al. “Large-scale interrogation of retinal cell functions by 1-photon light-sheet microscopy.Cell Rep Methods, vol. 3, no. 4, Apr. 2023, p. 100453. Pubmed, doi:10.1016/j.crmeth.2023.100453.
Roy S, Wang D, Rudzite AM, Perry B, Scalabrino ML, Thapa M, Gong Y, Sher A, Field GD. Large-scale interrogation of retinal cell functions by 1-photon light-sheet microscopy. Cell Rep Methods. 2023 Apr 24;3(4):100453.

Published In

Cell Rep Methods

DOI

EISSN

2667-2375

Publication Date

April 24, 2023

Volume

3

Issue

4

Start / End Page

100453

Location

United States

Related Subject Headings

  • Neurons
  • Microscopy
  • Law Enforcement
  • Coloring Agents
  • Calcium, Dietary