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Propagation of human prostate tissue from induced pluripotent stem cells.

Publication ,  Journal Article
Hepburn, AC; Curry, EL; Moad, M; Steele, RE; Franco, OE; Wilson, L; Singh, P; Buskin, A; Crawford, SE; Gaughan, L; Mills, IG; Hayward, SW ...
Published in: Stem Cells Transl Med
July 2020

Primary culture of human prostate organoids and patient-derived xenografts is inefficient and has limited access to clinical tissues. This hampers their use for translational study to identify new treatments. To overcome this, we established a complementary approach where rapidly proliferating and easily handled induced pluripotent stem cells enabled the generation of human prostate tissue in vivo and in vitro. By using a coculture technique with inductive urogenital sinus mesenchyme, we comprehensively recapitulated in situ 3D prostate histology, and overcame limitations in the primary culture of human prostate stem, luminal and neuroendocrine cells, as well as the stromal microenvironment. This model now unlocks new opportunities to undertake translational studies of benign and malignant prostate disease.

Duke Scholars

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Published In

Stem Cells Transl Med

DOI

EISSN

2157-6580

Publication Date

July 2020

Volume

9

Issue

7

Start / End Page

734 / 745

Location

England

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Prostate
  • Pregnancy
  • Mice, Nude
  • Mice
  • Male
  • Induced Pluripotent Stem Cells
  • Humans
  • Female
 

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Hepburn, A. C., Curry, E. L., Moad, M., Steele, R. E., Franco, O. E., Wilson, L., … Heer, R. (2020). Propagation of human prostate tissue from induced pluripotent stem cells. Stem Cells Transl Med, 9(7), 734–745. https://doi.org/10.1002/sctm.19-0286
Hepburn, Anastasia C., Emma L. Curry, Mohammad Moad, Rebecca E. Steele, Omar E. Franco, Laura Wilson, Parmveer Singh, et al. “Propagation of human prostate tissue from induced pluripotent stem cells.Stem Cells Transl Med 9, no. 7 (July 2020): 734–45. https://doi.org/10.1002/sctm.19-0286.
Hepburn AC, Curry EL, Moad M, Steele RE, Franco OE, Wilson L, et al. Propagation of human prostate tissue from induced pluripotent stem cells. Stem Cells Transl Med. 2020 Jul;9(7):734–45.
Hepburn, Anastasia C., et al. “Propagation of human prostate tissue from induced pluripotent stem cells.Stem Cells Transl Med, vol. 9, no. 7, July 2020, pp. 734–45. Pubmed, doi:10.1002/sctm.19-0286.
Hepburn AC, Curry EL, Moad M, Steele RE, Franco OE, Wilson L, Singh P, Buskin A, Crawford SE, Gaughan L, Mills IG, Hayward SW, Robson CN, Heer R. Propagation of human prostate tissue from induced pluripotent stem cells. Stem Cells Transl Med. 2020 Jul;9(7):734–745.

Published In

Stem Cells Transl Med

DOI

EISSN

2157-6580

Publication Date

July 2020

Volume

9

Issue

7

Start / End Page

734 / 745

Location

England

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Prostate
  • Pregnancy
  • Mice, Nude
  • Mice
  • Male
  • Induced Pluripotent Stem Cells
  • Humans
  • Female