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Thrombospondin-1, vascular endothelial growth factor and fibroblast growth factor-2 are key functional regulators of angiogenesis in the prostate.

Publication ,  Journal Article
Doll, JA; Reiher, FK; Crawford, SE; Pins, MR; Campbell, SC; Bouck, NP
Published in: Prostate
December 1, 2001

BACKGROUND: Prostate cells secrete many molecules capable of regulating angiogenesis; however, which of these actually function as essential regulators of neovascularization is not yet clear. METHODS: Functional angiogenic mediators secreted by normal and diseased prostate cells were identified using an in vitro angiogenesis assay. These factors were quantified by immunoblot or ELISA and localized in tissue by immunohistochemistry. RESULTS: Normal prostate epithelial cell secretions were anti-angiogenic due to inhibitory thrombospondin-1 (TSP-1) whereas this inhibitor was decreased in the pro-angiogenic secretions derived from benign prostatic hyperplasia (BPH) and cancer cells. This pro-angiogenic activity depended primarily on fibroblast growth factor-2 (FGF-2) and/or vascular endothelial growth factor (VEGF) whose secretion was increased. Immunolocalization studies confirmed that the changes detected in vitro also occurred in vivo. CONCLUSIONS: During disease progression in the prostate, production of TSP-1, the major inhibitor, is down-regulated while that of stimulatory FGF-2 and/or VEGF rise, leading to the induction of the new vessels necessary to support tumor growth.

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Published In

Prostate

DOI

ISSN

0270-4137

Publication Date

December 1, 2001

Volume

49

Issue

4

Start / End Page

293 / 305

Location

United States

Related Subject Headings

  • Vascular Endothelial Growth Factors
  • Vascular Endothelial Growth Factor A
  • Tumor Cells, Cultured
  • Thrombospondin 1
  • Prostatic Neoplasms
  • Prostatic Hyperplasia
  • Prostate
  • Oncology & Carcinogenesis
  • Neovascularization, Physiologic
  • Neovascularization, Pathologic
 

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Doll, J. A., Reiher, F. K., Crawford, S. E., Pins, M. R., Campbell, S. C., & Bouck, N. P. (2001). Thrombospondin-1, vascular endothelial growth factor and fibroblast growth factor-2 are key functional regulators of angiogenesis in the prostate. Prostate, 49(4), 293–305. https://doi.org/10.1002/pros.10025
Doll, J. A., F. K. Reiher, S. E. Crawford, M. R. Pins, S. C. Campbell, and N. P. Bouck. “Thrombospondin-1, vascular endothelial growth factor and fibroblast growth factor-2 are key functional regulators of angiogenesis in the prostate.Prostate 49, no. 4 (December 1, 2001): 293–305. https://doi.org/10.1002/pros.10025.
Doll JA, Reiher FK, Crawford SE, Pins MR, Campbell SC, Bouck NP. Thrombospondin-1, vascular endothelial growth factor and fibroblast growth factor-2 are key functional regulators of angiogenesis in the prostate. Prostate. 2001 Dec 1;49(4):293–305.
Doll, J. A., et al. “Thrombospondin-1, vascular endothelial growth factor and fibroblast growth factor-2 are key functional regulators of angiogenesis in the prostate.Prostate, vol. 49, no. 4, Dec. 2001, pp. 293–305. Pubmed, doi:10.1002/pros.10025.
Doll JA, Reiher FK, Crawford SE, Pins MR, Campbell SC, Bouck NP. Thrombospondin-1, vascular endothelial growth factor and fibroblast growth factor-2 are key functional regulators of angiogenesis in the prostate. Prostate. 2001 Dec 1;49(4):293–305.
Journal cover image

Published In

Prostate

DOI

ISSN

0270-4137

Publication Date

December 1, 2001

Volume

49

Issue

4

Start / End Page

293 / 305

Location

United States

Related Subject Headings

  • Vascular Endothelial Growth Factors
  • Vascular Endothelial Growth Factor A
  • Tumor Cells, Cultured
  • Thrombospondin 1
  • Prostatic Neoplasms
  • Prostatic Hyperplasia
  • Prostate
  • Oncology & Carcinogenesis
  • Neovascularization, Physiologic
  • Neovascularization, Pathologic