The use of flow cytometry as a tool for monitoring filament formation of fungi.

Journal Article

Flow cytometry (FC) has the ability to discriminate a variety of cell parameters including cell size and complexity, and fluorescence intensity. As yeast cells or fungal spores germinate they undergo a morphological transformation from round oval shaped cells to elongate filamentous forms. To date, monitoring these events has been performed using microscopic examination. Microscopic examination is a labor intensive process that examines a very small percentage of the total cell population. We have developed a method using FC that is rapid, simple to perform, and reproducible. The major advantages of FC include analysis of a larger number of cells, increased objectivity due to nonselective measurements of all cells in the population studied, and the computer related data analysis capability of the flow cytometer.

Full Text

Duke Authors

Cited Authors

  • Hopfer, RL; Sparks, SD; Cox, GM

Published Date

  • February 2001

Published In

Volume / Issue

  • 39 / 1

Start / End Page

  • 103 - 107

PubMed ID

  • 11270396

International Standard Serial Number (ISSN)

  • 1369-3786

Language

  • eng

Conference Location

  • England