Strain-dependent effects of environmental signals on the production of extracellular phospholipase by Cryptococcus neoformans.


Journal Article

Extracellular phospholipase (PL) activities comprising phospholipase B, lysophospholipase and lysophospholipase transacylase have been identified in culture supernatants of Cryptococcus neoformans and contribute to virulence. We found that PL production was optimal after fungal growth at 30 degrees C and secretion at 37 degrees C for all six C. neoformans isolates studied (four C. neoformans var. neoformans and two C. neoformans var. gattii). No increase in PL activity was found in one strain, NU-2, in low iron or tissue culture media, conditions where upregulation of other virulence factors has been reported. The most virulent strains in an intravenous mouse model of infection were best able to produce PL at growth and secretion temperatures of 37 degrees C, in tissue culture media and under assay conditions of pH 7.0.

Full Text

Duke Authors

Cited Authors

  • Wright, LC; Chen, SCA; Wilson, CF; Simpanya, MF; Blackstock, R; Cox, GM; Murphy, JW; Sorrell, TC

Published Date

  • April 9, 2002

Published In

Volume / Issue

  • 209 / 2

Start / End Page

  • 175 - 181

PubMed ID

  • 12007802

Pubmed Central ID

  • 12007802

International Standard Serial Number (ISSN)

  • 0378-1097

Digital Object Identifier (DOI)

  • 10.1111/j.1574-6968.2002.tb11128.x


  • eng

Conference Location

  • England