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Studies of complement-activating antibodies in the SIV/macaque model of acute primary infection and vaccine protection.

Publication ,  Journal Article
Montefiori, DC; Reimann, KA; Letvin, NL; Zhou, J; Hu, SL
Published in: AIDS Res Hum Retroviruses
August 1995

Questions regarding the potential impact of complement-activating antibodies on lentivirus pathogenesis and vaccine development were addressed in the SIV/macaque model by evaluating sera for activity related to complement-mediated, antibody-dependent enhancement (C'-ADE) of SIV infection in vitro. C'-ADE activity in sera obtained during acute primary infection in macaques inoculated with SIVmac251 appeared before neutralizing antibodies and coincided with the initial peak and decline of plasma antigenemia. The power of C'-ADE activity (i.e., virus production measured by p24 immunoassay) decreased as titers of neutralizing antibodies increased in these animals, suggesting a balance in the net effect between C'-ADE and neutralizing activities in vitro. Antibodies with C'-ADE activity were also induced in macaques immunized with live-attenuated SIVmac239/nef-deletion or primed with recombinant SIVmne gp120 vaccinia virus and boosted with SIVmne rgp160. The titer (i.e., last serum dilution to show enhancement), peak (i.e., serum dilution producing the greatest enhancement as measured by p24 production), and power (i.e., magnitude of p24 production at the peak titer) of C'-ADE activity in sera obtained from vaccinated macaques on the day of challenge were comparable to those of sera from infected macaques and showed no correlation with vaccine outcome, where some protected animals had C'-ADE profiles that resembled those of unprotected animals. The results of these studies suggest that antibodies having C'-ADE activity in vitro could contribute to virus replication or, alternatively, to virus clearance during the acute stage of SIV infection in macaques.(ABSTRACT TRUNCATED AT 250 WORDS)

Duke Scholars

Published In

AIDS Res Hum Retroviruses

DOI

ISSN

0889-2229

Publication Date

August 1995

Volume

11

Issue

8

Start / End Page

963 / 970

Location

United States

Related Subject Headings

  • Virology
  • Viral Vaccines
  • Viral Envelope Proteins
  • Vaccination
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Simian Acquired Immunodeficiency Syndrome
  • Recombinant Proteins
  • Macaca
  • Complement Activation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Montefiori, D. C., Reimann, K. A., Letvin, N. L., Zhou, J., & Hu, S. L. (1995). Studies of complement-activating antibodies in the SIV/macaque model of acute primary infection and vaccine protection. AIDS Res Hum Retroviruses, 11(8), 963–970. https://doi.org/10.1089/aid.1995.11.963
Montefiori, D. C., K. A. Reimann, N. L. Letvin, J. Zhou, and S. L. Hu. “Studies of complement-activating antibodies in the SIV/macaque model of acute primary infection and vaccine protection.AIDS Res Hum Retroviruses 11, no. 8 (August 1995): 963–70. https://doi.org/10.1089/aid.1995.11.963.
Montefiori DC, Reimann KA, Letvin NL, Zhou J, Hu SL. Studies of complement-activating antibodies in the SIV/macaque model of acute primary infection and vaccine protection. AIDS Res Hum Retroviruses. 1995 Aug;11(8):963–70.
Montefiori, D. C., et al. “Studies of complement-activating antibodies in the SIV/macaque model of acute primary infection and vaccine protection.AIDS Res Hum Retroviruses, vol. 11, no. 8, Aug. 1995, pp. 963–70. Pubmed, doi:10.1089/aid.1995.11.963.
Montefiori DC, Reimann KA, Letvin NL, Zhou J, Hu SL. Studies of complement-activating antibodies in the SIV/macaque model of acute primary infection and vaccine protection. AIDS Res Hum Retroviruses. 1995 Aug;11(8):963–970.
Journal cover image

Published In

AIDS Res Hum Retroviruses

DOI

ISSN

0889-2229

Publication Date

August 1995

Volume

11

Issue

8

Start / End Page

963 / 970

Location

United States

Related Subject Headings

  • Virology
  • Viral Vaccines
  • Viral Envelope Proteins
  • Vaccination
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Simian Acquired Immunodeficiency Syndrome
  • Recombinant Proteins
  • Macaca
  • Complement Activation