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In vitro and in vivo activity of murine lymphokine-activated killer cells after cryopreservation.

Publication ,  Journal Article
Schmidt-Wolf, IG; Aihara, M; Negrin, RS; Blume, KG; Chao, NJ
Published in: Transfusion
January 1992

The in vitro and in vivo effects of cryopreservation on the cytotoxic activity of murine lymphokine-activated killer (LAK) cells were studied. LAK cells were generated by incubation of spleen lymphocytes of BALB/c mice for 3 days with recombinant interleukin-2 (rIL-2) and subsequent cryopreservation. Cytotoxicity was determined in a 51Cr release assay. After thawing, cytotoxic activity was reduced (40.4% 51Cr release at an effector:target cell ratio of 40:1 as compared to 68.5% 51Cr release before freezing) and could be restored to precryopreserved levels by reincubation with rIL-2 for 2 days after thawing (78.8% 51Cr release). These cells were then tested in BALB/c mice injected with RAW 112 cells, a pre-B-cell lymphoma line. The results demonstrate that the survival rate of mice injected with cryopreserved and restimulated LAK cells (50% survival greater than 180 days after injection) did not differ significantly from that of mice injected with fresh unfrozen LAK cells (60% survival greater than 120 days, 50% survival greater than 180 days). Cryopreserved LAK cells have potential use in adoptive immunotherapy.

Duke Scholars

Published In

Transfusion

DOI

ISSN

0041-1132

Publication Date

January 1992

Volume

32

Issue

1

Start / End Page

42 / 45

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Time Factors
  • Recombinant Proteins
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Lymphoma, B-Cell
  • Killer Cells, Lymphokine-Activated
  • Interleukin-2
  • Cryopreservation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Schmidt-Wolf, I. G., Aihara, M., Negrin, R. S., Blume, K. G., & Chao, N. J. (1992). In vitro and in vivo activity of murine lymphokine-activated killer cells after cryopreservation. Transfusion, 32(1), 42–45. https://doi.org/10.1046/j.1537-2995.1992.32192116431.x
Schmidt-Wolf, I. G., M. Aihara, R. S. Negrin, K. G. Blume, and N. J. Chao. “In vitro and in vivo activity of murine lymphokine-activated killer cells after cryopreservation.Transfusion 32, no. 1 (January 1992): 42–45. https://doi.org/10.1046/j.1537-2995.1992.32192116431.x.
Schmidt-Wolf IG, Aihara M, Negrin RS, Blume KG, Chao NJ. In vitro and in vivo activity of murine lymphokine-activated killer cells after cryopreservation. Transfusion. 1992 Jan;32(1):42–5.
Schmidt-Wolf, I. G., et al. “In vitro and in vivo activity of murine lymphokine-activated killer cells after cryopreservation.Transfusion, vol. 32, no. 1, Jan. 1992, pp. 42–45. Pubmed, doi:10.1046/j.1537-2995.1992.32192116431.x.
Schmidt-Wolf IG, Aihara M, Negrin RS, Blume KG, Chao NJ. In vitro and in vivo activity of murine lymphokine-activated killer cells after cryopreservation. Transfusion. 1992 Jan;32(1):42–45.
Journal cover image

Published In

Transfusion

DOI

ISSN

0041-1132

Publication Date

January 1992

Volume

32

Issue

1

Start / End Page

42 / 45

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Time Factors
  • Recombinant Proteins
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Lymphoma, B-Cell
  • Killer Cells, Lymphokine-Activated
  • Interleukin-2
  • Cryopreservation