In vitro and in vivo activity of murine lymphokine-activated killer cells after cryopreservation.

Published

Journal Article

The in vitro and in vivo effects of cryopreservation on the cytotoxic activity of murine lymphokine-activated killer (LAK) cells were studied. LAK cells were generated by incubation of spleen lymphocytes of BALB/c mice for 3 days with recombinant interleukin-2 (rIL-2) and subsequent cryopreservation. Cytotoxicity was determined in a 51Cr release assay. After thawing, cytotoxic activity was reduced (40.4% 51Cr release at an effector:target cell ratio of 40:1 as compared to 68.5% 51Cr release before freezing) and could be restored to precryopreserved levels by reincubation with rIL-2 for 2 days after thawing (78.8% 51Cr release). These cells were then tested in BALB/c mice injected with RAW 112 cells, a pre-B-cell lymphoma line. The results demonstrate that the survival rate of mice injected with cryopreserved and restimulated LAK cells (50% survival greater than 180 days after injection) did not differ significantly from that of mice injected with fresh unfrozen LAK cells (60% survival greater than 120 days, 50% survival greater than 180 days). Cryopreserved LAK cells have potential use in adoptive immunotherapy.

Full Text

Duke Authors

Cited Authors

  • Schmidt-Wolf, IG; Aihara, M; Negrin, RS; Blume, KG; Chao, NJ

Published Date

  • January 1, 1992

Published In

Volume / Issue

  • 32 / 1

Start / End Page

  • 42 - 45

PubMed ID

  • 1731434

Pubmed Central ID

  • 1731434

International Standard Serial Number (ISSN)

  • 0041-1132

Language

  • eng

Conference Location

  • United States