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Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand.

Publication ,  Journal Article
Bowen, MA; Patel, DD; Li, X; Modrell, B; Malacko, AR; Wang, WC; Marquardt, H; Neubauer, M; Pesando, JM; Francke, U
Published in: J Exp Med
June 1, 1995

Antibody-blocking studies have demonstrated the role of CD6 in thymocyte-thymic epithelial (TE) cell adhesion. Here we report that CD6 expressed by COS cells mediates adhesion to TE cells and that this interaction is specifically blocked with an anti-CD6 monoclonal antibody (mAb) or with a mAb (J4-81) that recognized a TE cell antigen. We isolated and expressed a cDNA clone encoding this antigen and show that COS cells transfected with this cDNA bind a CD6 immunoglobulin fusion protein (CD6-Rg). This antigen, which we named ALCAM (activated leukocyte-cell adhesion molecule) because of its expression on activated leukocytes, appears to be the human homologue of the chicken neural adhesion molecule BEN/SC-1/DM-GRASP. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. We prepared an ALCAM-Rg fusion protein and showed that it binds to COS cell transfectants expressing CD6, demonstrating that ALCAM is a CD6 ligand. The observations that ALCAM is also expressed by activated leukocytes and that both ALCAM and CD6 are expressed in the brain suggest that ALCAM-CD6 interactions may play a role in the binding of T and B cells to activated leukocytes, as well as in interactions between cells of the nervous system.

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Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

June 1, 1995

Volume

181

Issue

6

Start / End Page

2213 / 2220

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Thymus Gland
  • T-Lymphocytes
  • Sequence Homology, Amino Acid
  • Recombinant Fusion Proteins
  • Molecular Sequence Data
  • Ligands
  • Karyotyping
  • Immunology
 

Citation

APA
Chicago
ICMJE
MLA
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Bowen, M. A., Patel, D. D., Li, X., Modrell, B., Malacko, A. R., Wang, W. C., … Francke, U. (1995). Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand. J Exp Med, 181(6), 2213–2220. https://doi.org/10.1084/jem.181.6.2213
Bowen, M. A., D. D. Patel, X. Li, B. Modrell, A. R. Malacko, W. C. Wang, H. Marquardt, M. Neubauer, J. M. Pesando, and U. Francke. “Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand.J Exp Med 181, no. 6 (June 1, 1995): 2213–20. https://doi.org/10.1084/jem.181.6.2213.
Bowen MA, Patel DD, Li X, Modrell B, Malacko AR, Wang WC, et al. Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand. J Exp Med. 1995 Jun 1;181(6):2213–20.
Bowen, M. A., et al. “Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand.J Exp Med, vol. 181, no. 6, June 1995, pp. 2213–20. Pubmed, doi:10.1084/jem.181.6.2213.
Bowen MA, Patel DD, Li X, Modrell B, Malacko AR, Wang WC, Marquardt H, Neubauer M, Pesando JM, Francke U. Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand. J Exp Med. 1995 Jun 1;181(6):2213–2220.

Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

June 1, 1995

Volume

181

Issue

6

Start / End Page

2213 / 2220

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Thymus Gland
  • T-Lymphocytes
  • Sequence Homology, Amino Acid
  • Recombinant Fusion Proteins
  • Molecular Sequence Data
  • Ligands
  • Karyotyping
  • Immunology