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Positive and negative elements regulate human interleukin 3 expression.

Publication ,  Journal Article
Mathey-Prevot, B; Andrews, NC; Murphy, HS; Kreissman, SG; Nathan, DG
Published in: Proc Natl Acad Sci U S A
July 1990

The human interleukin 3 (IL-3) promoter is comprised of several cis-acting DNA sequences that modulate T-cell expression of IL-3. These are located within 315 nucleotides upstream of the mRNA start site. Transient expression of reporter genes linked to serially deleted sequences of the IL-3 promoter has allowed mapping of two activator sequences and an interposed repressor sequence. The proximal regulatory region is specific to IL-3 and prerequisite for efficient transcription. Its effect is enhanced by a second, more distal activating sequence consisting of an AP-1 binding site. Between the two activators lies a transcriptional silencer, which is a potent repressor in the absence of the AP-1 site. DNA-nuclear protein binding experiments demonstrate specific complex formation within each of these functional regions. Thus, both positive and negative regulatory elements appear to control expression of the human IL-3 gene in activated T cells.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

July 1990

Volume

87

Issue

13

Start / End Page

5046 / 5050

Location

United States

Related Subject Headings

  • Transfection
  • T-Lymphocytes
  • Ribonucleases
  • RNA
  • Promoter Regions, Genetic
  • Oligonucleotide Probes
  • Molecular Sequence Data
  • Lymphocyte Activation
  • Interleukin-3
  • Humans
 

Citation

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ICMJE
MLA
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Mathey-Prevot, B., Andrews, N. C., Murphy, H. S., Kreissman, S. G., & Nathan, D. G. (1990). Positive and negative elements regulate human interleukin 3 expression. Proc Natl Acad Sci U S A, 87(13), 5046–5050. https://doi.org/10.1073/pnas.87.13.5046
Mathey-Prevot, B., N. C. Andrews, H. S. Murphy, S. G. Kreissman, and D. G. Nathan. “Positive and negative elements regulate human interleukin 3 expression.Proc Natl Acad Sci U S A 87, no. 13 (July 1990): 5046–50. https://doi.org/10.1073/pnas.87.13.5046.
Mathey-Prevot B, Andrews NC, Murphy HS, Kreissman SG, Nathan DG. Positive and negative elements regulate human interleukin 3 expression. Proc Natl Acad Sci U S A. 1990 Jul;87(13):5046–50.
Mathey-Prevot, B., et al. “Positive and negative elements regulate human interleukin 3 expression.Proc Natl Acad Sci U S A, vol. 87, no. 13, July 1990, pp. 5046–50. Pubmed, doi:10.1073/pnas.87.13.5046.
Mathey-Prevot B, Andrews NC, Murphy HS, Kreissman SG, Nathan DG. Positive and negative elements regulate human interleukin 3 expression. Proc Natl Acad Sci U S A. 1990 Jul;87(13):5046–5050.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

July 1990

Volume

87

Issue

13

Start / End Page

5046 / 5050

Location

United States

Related Subject Headings

  • Transfection
  • T-Lymphocytes
  • Ribonucleases
  • RNA
  • Promoter Regions, Genetic
  • Oligonucleotide Probes
  • Molecular Sequence Data
  • Lymphocyte Activation
  • Interleukin-3
  • Humans