Generating monoclonal antibodies to neuronal antigens.


Journal Article

Methods for generating monoclonal antibodies directed to the functional sites of neuronal antigens are reviewed. These methods include optimal antigen preparation and presentation as well as selective targeting and manipulation of the antigenic response. We describe our use of the immunosuppressant drug, cyclophosphamide, to produce a selective immune response to rare, poorly immunogenic, or actively suppressed antigens. These techniques allow us to generate antibodies to the functional sites of neuronal antigens, such as cell surface molecules. Such antibodies are directed to complex carbohydrates, proteins, protein complexes and glycolipids that form the active site of neuronal antigens. We can use these antibodies in the molecular dissection of functional active sites that are inaccessible to genetic manipulation. These techniques favor the generation of antibodies that can be used to understand and manipulate neuronal cellular activity.

Full Text

Duke Authors

Cited Authors

  • Riggott, MJ; Matthew, WD

Published Date

  • October 1996

Published In

Volume / Issue

  • 68 / 2

Start / End Page

  • 235 - 245

PubMed ID

  • 8912196

Pubmed Central ID

  • 8912196

International Standard Serial Number (ISSN)

  • 0165-0270

Digital Object Identifier (DOI)

  • 10.1016/0165-0270(96)00085-4


  • eng

Conference Location

  • Netherlands