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Generating monoclonal antibodies to neuronal antigens.

Publication ,  Journal Article
Riggott, MJ; Matthew, WD
Published in: J Neurosci Methods
October 1996

Methods for generating monoclonal antibodies directed to the functional sites of neuronal antigens are reviewed. These methods include optimal antigen preparation and presentation as well as selective targeting and manipulation of the antigenic response. We describe our use of the immunosuppressant drug, cyclophosphamide, to produce a selective immune response to rare, poorly immunogenic, or actively suppressed antigens. These techniques allow us to generate antibodies to the functional sites of neuronal antigens, such as cell surface molecules. Such antibodies are directed to complex carbohydrates, proteins, protein complexes and glycolipids that form the active site of neuronal antigens. We can use these antibodies in the molecular dissection of functional active sites that are inaccessible to genetic manipulation. These techniques favor the generation of antibodies that can be used to understand and manipulate neuronal cellular activity.

Duke Scholars

Published In

J Neurosci Methods

DOI

ISSN

0165-0270

Publication Date

October 1996

Volume

68

Issue

2

Start / End Page

235 / 245

Location

Netherlands

Related Subject Headings

  • Rats
  • Neurology & Neurosurgery
  • Immunohistochemistry
  • Cyclophosphamide
  • Cerebral Cortex
  • B-Lymphocytes
  • Antigens
  • Antibodies, Monoclonal
  • Animals
  • 3209 Neurosciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Riggott, M. J., & Matthew, W. D. (1996). Generating monoclonal antibodies to neuronal antigens. J Neurosci Methods, 68(2), 235–245. https://doi.org/10.1016/0165-0270(96)00085-4
Riggott, M. J., and W. D. Matthew. “Generating monoclonal antibodies to neuronal antigens.J Neurosci Methods 68, no. 2 (October 1996): 235–45. https://doi.org/10.1016/0165-0270(96)00085-4.
Riggott MJ, Matthew WD. Generating monoclonal antibodies to neuronal antigens. J Neurosci Methods. 1996 Oct;68(2):235–45.
Riggott, M. J., and W. D. Matthew. “Generating monoclonal antibodies to neuronal antigens.J Neurosci Methods, vol. 68, no. 2, Oct. 1996, pp. 235–45. Pubmed, doi:10.1016/0165-0270(96)00085-4.
Riggott MJ, Matthew WD. Generating monoclonal antibodies to neuronal antigens. J Neurosci Methods. 1996 Oct;68(2):235–245.
Journal cover image

Published In

J Neurosci Methods

DOI

ISSN

0165-0270

Publication Date

October 1996

Volume

68

Issue

2

Start / End Page

235 / 245

Location

Netherlands

Related Subject Headings

  • Rats
  • Neurology & Neurosurgery
  • Immunohistochemistry
  • Cyclophosphamide
  • Cerebral Cortex
  • B-Lymphocytes
  • Antigens
  • Antibodies, Monoclonal
  • Animals
  • 3209 Neurosciences