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Cyclophosphamide treatment used to manipulate the immune response for the production of monoclonal antibodies.

Publication ,  Journal Article
Matthew, WD; Sandrock, AW
Published in: J Immunol Methods
June 26, 1987

After immunization with a complex mixture of antigens, a considerable bias toward obtaining monoclonal antibodies to immunodominant determinants exists. By selectively killing antigen-stimulated lymphocytes, the cytotoxic drug cyclophosphamide can be used to manipulate the bias of the normal immune response. Cyclophosphamide has been used to tolerize mice to one set of antigens followed by immunization with a similar but slightly different set of antigens. This approach yields an enhanced frequency of antibodies that distinguish the two sets of antigens. Cyclophosphamide treatment has also allowed us to produce monoclonal antibodies to weakly immunogenic glycosaminoglycans and to obtain a high frequency of apparently anti-idiotypic antibodies.

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Published In

J Immunol Methods

DOI

ISSN

0022-1759

Publication Date

June 26, 1987

Volume

100

Issue

1-2

Start / End Page

73 / 82

Location

Netherlands

Related Subject Headings

  • Spinal Cord
  • Sciatic Nerve
  • Rats
  • Polysaccharides
  • Mice, Inbred BALB C
  • Mice
  • Immunology
  • Immunoglobulin Idiotypes
  • Immunization
  • Immune Tolerance
 

Citation

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Matthew, W. D., & Sandrock, A. W. (1987). Cyclophosphamide treatment used to manipulate the immune response for the production of monoclonal antibodies. J Immunol Methods, 100(1–2), 73–82. https://doi.org/10.1016/0022-1759(87)90174-8
Matthew, W. D., and A. W. Sandrock. “Cyclophosphamide treatment used to manipulate the immune response for the production of monoclonal antibodies.J Immunol Methods 100, no. 1–2 (June 26, 1987): 73–82. https://doi.org/10.1016/0022-1759(87)90174-8.
Matthew WD, Sandrock AW. Cyclophosphamide treatment used to manipulate the immune response for the production of monoclonal antibodies. J Immunol Methods. 1987 Jun 26;100(1–2):73–82.
Matthew, W. D., and A. W. Sandrock. “Cyclophosphamide treatment used to manipulate the immune response for the production of monoclonal antibodies.J Immunol Methods, vol. 100, no. 1–2, June 1987, pp. 73–82. Pubmed, doi:10.1016/0022-1759(87)90174-8.
Matthew WD, Sandrock AW. Cyclophosphamide treatment used to manipulate the immune response for the production of monoclonal antibodies. J Immunol Methods. 1987 Jun 26;100(1–2):73–82.
Journal cover image

Published In

J Immunol Methods

DOI

ISSN

0022-1759

Publication Date

June 26, 1987

Volume

100

Issue

1-2

Start / End Page

73 / 82

Location

Netherlands

Related Subject Headings

  • Spinal Cord
  • Sciatic Nerve
  • Rats
  • Polysaccharides
  • Mice, Inbred BALB C
  • Mice
  • Immunology
  • Immunoglobulin Idiotypes
  • Immunization
  • Immune Tolerance