Cyclophosphamide treatment used to manipulate the immune response for the production of monoclonal antibodies.
After immunization with a complex mixture of antigens, a considerable bias toward obtaining monoclonal antibodies to immunodominant determinants exists. By selectively killing antigen-stimulated lymphocytes, the cytotoxic drug cyclophosphamide can be used to manipulate the bias of the normal immune response. Cyclophosphamide has been used to tolerize mice to one set of antigens followed by immunization with a similar but slightly different set of antigens. This approach yields an enhanced frequency of antibodies that distinguish the two sets of antigens. Cyclophosphamide treatment has also allowed us to produce monoclonal antibodies to weakly immunogenic glycosaminoglycans and to obtain a high frequency of apparently anti-idiotypic antibodies.
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- Spinal Cord
- Sciatic Nerve
- Rats
- Polysaccharides
- Mice, Inbred BALB C
- Mice
- Immunology
- Immunoglobulin Idiotypes
- Immunization
- Immune Tolerance
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Spinal Cord
- Sciatic Nerve
- Rats
- Polysaccharides
- Mice, Inbred BALB C
- Mice
- Immunology
- Immunoglobulin Idiotypes
- Immunization
- Immune Tolerance