Interaction of apolipoprotein E with laminin increases neuronal adhesion and alters neurite morphology.

Published

Journal Article

The extracellular matrix protein laminin profoundly affects neuronal adhesion, spreading, differentiation, and growth by binding integrin-type cell surface receptors. Laminin binds other basement membrane components, including heparan sulfate proteoglycans. Apolipoprotein E (apoE) also binds basement membrane and heparan sulfate proteoglycans and colocalizes with s-laminin in the neuromuscular junction. We sought to determine whether apoE interacts with laminin and whether, as a result, apoE alters the regulation of neuronal adhesion and differentiation by laminin. We demonstrate high-avidity interaction between apoE and laminin in vitro. Compared to laminin alone, a laminin-apoE substrate produces increased numbers of live, attached hippocampal neurons in culture. In addition, neurons grown on laminin-apoE substrates have larger growth cones, increased neuritic branching, and flattened cell bodies compared to neurons grown on laminin alone. ApoE may be important in the development and maintenance of neurons in the central nervous system by regulating interactions between the neuron and the extracellular matrix.

Full Text

Duke Authors

Cited Authors

  • Huang, DY; Weisgraber, KH; Strittmatter, WJ; Matthew, WD

Published Date

  • December 1995

Published In

Volume / Issue

  • 136 / 2

Start / End Page

  • 251 - 257

PubMed ID

  • 7498414

Pubmed Central ID

  • 7498414

International Standard Serial Number (ISSN)

  • 0014-4886

Digital Object Identifier (DOI)

  • 10.1006/exnr.1995.1101

Language

  • eng

Conference Location

  • United States