An in vitro neurite-promoting antigen functions in axonal regeneration in vivo.

Journal Article (Journal Article)

The function of the neurite growth-promoting antigen INO has been tested in an in vivo neurite regeneration system, the rat iris. The sympathetic innervation of the irides was removed by a single systemic injection of 6-hydroxydopamine. The subsequent regeneration of sympathetic axons into the iris of one eye bathed by the INO antibody, which inhibits neurite growth in vitro, was compared with the regrowth of sympathetic axons into the iris of the animal's other eye, which contained control antibody. Antibodies were released within the eye by implanted hybridoma cells. Neurite regeneration was measured by assaying [3H]norepinephrine uptake into freshly explained irides. The blockage of the function of the INO antigen by the antibody resulted in a decreased rate of axonal regeneration, thus suggesting the involvement of the INO antigen in the process of neurite regeneration in vivo.

Full Text

Duke Authors

Cited Authors

  • Sandrock, AW; Matthew, WD

Published Date

  • September 25, 1987

Published In

Volume / Issue

  • 237 / 4822

Start / End Page

  • 1605 - 1608

PubMed ID

  • 3306923

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.3306923


  • eng

Conference Location

  • United States