Transcription of H-2 and Qa genes in embryonic and adult mice.
Journal Article (Journal Article)
We have isolated cDNAs for three class I genes from an 8.5-day C57Bl/6 mouse embryo cDNA library. Two of these cDNAs encode the classical transplantation antigens, H-2Kb and H-2Db. The third is a novelly spliced form of a Qa region gene, Q9, which lacks several exons including that encoding the transmembrane domain. Since this mRNA is detected on membrane-bound polysomes it may encode a secreted protein. Transcripts for these three genes and the pseudo-allele of Q9, Q7, were present at very low levels in the 8.5-day and 14.5-day embryo and in the 2-day neonate, compared to most adult tissues. Q10, the liver-specific secreted class I gene product in adults, is preferentially expressed in yolk sac and in fetal liver. We found no embryo-specific class I gene expression.
Full Text
Duke Authors
Cited Authors
- Fahrner, K; Hogan, BL; Flavell, RA
Published Date
- May 1987
Published In
Volume / Issue
- 6 / 5
Start / End Page
- 1265 - 1271
PubMed ID
- 3608979
Pubmed Central ID
- PMC553928
International Standard Serial Number (ISSN)
- 0261-4189
Digital Object Identifier (DOI)
- 10.1002/j.1460-2075.1987.tb02363.x
Language
- eng
Conference Location
- England