Localization of fibronectin, laminin-entactin, and entactin in Reichert's membrane by immunoelectron microscopy.
Journal Article (Journal Article)
Immunoelectron microscopy using protein A-colloidal gold complexes of different sizes was used to study the relative distribution of extracellular matrix glycoproteins within Reichert's membrane (RM) of 13.5-day mouse embryos. Labelling for fibronectin was distributed asymmetrically; the highest concentration occurring in the outermost layer adjacent to the trophoblast cells and negligible labelling in the inner matrix, beneath the parietal endoderm cells. Within the main body of the membrane, fibronectin was concentrated in discrete electron-opaque deposits. Antibodies raised against the native complex between laminin and entactin , and against entactin alone labelled the RM more uniformly.
Full Text
Duke Authors
Cited Authors
- Semoff, S; Hogan, BL; Hopkins, CR
Published Date
- 1982
Published In
Volume / Issue
- 1 / 10
Start / End Page
- 1171 - 1175
PubMed ID
- 7188248
Pubmed Central ID
- PMC553185
International Standard Serial Number (ISSN)
- 0261-4189
Digital Object Identifier (DOI)
- 10.1002/j.1460-2075.1982.tb00009.x
Language
- eng
Conference Location
- England