Effect of Steel factor and leukaemia inhibitory factor on murine primordial germ cells in culture.
Journal Article (Journal Article)
Despite the importance of germ cells to the survival of species, surprisingly little is known about their embryological origin, proliferation, migration and entry into mitotic arrest or meiosis. Mutations in the murine Dominant White Spotting (W) and Steel genes, which respectively encode the c-kit tyrosine kinase receptor and the c-kit ligand (or Steel factor), impair the development of primordial germ cells (PGCs) in vivo, as well as haematopoietic stem cells and neural crest-derived melanoblasts. Here we use a monoclonal antibody against c-kit tyrosine kinase receptor and recombinant Steel factor to study the c-kit receptor-ligand system in cultured PGCs. In addition, we show that leukaemia inhibitory factor (also known as differentiation inhibitory activity), a factor secreted by STO fibroblasts, can stimulate proliferation of primordial germ cells in vitro.
Full Text
Duke Authors
Cited Authors
- Matsui, Y; Toksoz, D; Nishikawa, S; Williams, D; Zsebo, K; Hogan, BL
Published Date
- October 24, 1991
Published In
Volume / Issue
- 353 / 6346
Start / End Page
- 750 - 752
PubMed ID
- 1719421
International Standard Serial Number (ISSN)
- 0028-0836
Digital Object Identifier (DOI)
- 10.1038/353750a0
Language
- eng
Conference Location
- England