Effect of Steel factor and leukaemia inhibitory factor on murine primordial germ cells in culture.

Journal Article

Despite the importance of germ cells to the survival of species, surprisingly little is known about their embryological origin, proliferation, migration and entry into mitotic arrest or meiosis. Mutations in the murine Dominant White Spotting (W) and Steel genes, which respectively encode the c-kit tyrosine kinase receptor and the c-kit ligand (or Steel factor), impair the development of primordial germ cells (PGCs) in vivo, as well as haematopoietic stem cells and neural crest-derived melanoblasts. Here we use a monoclonal antibody against c-kit tyrosine kinase receptor and recombinant Steel factor to study the c-kit receptor-ligand system in cultured PGCs. In addition, we show that leukaemia inhibitory factor (also known as differentiation inhibitory activity), a factor secreted by STO fibroblasts, can stimulate proliferation of primordial germ cells in vitro.

Full Text

Duke Authors

Cited Authors

  • Matsui, Y; Toksoz, D; Nishikawa, S; Nishikawa, S; Williams, D; Zsebo, K; Hogan, BL

Published Date

  • October 24, 1991

Published In

Volume / Issue

  • 353 / 6346

Start / End Page

  • 750 - 752

PubMed ID

  • 1719421

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/353750a0

Language

  • eng

Conference Location

  • England