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The isolation and characterization of a novel cDNA demonstrating an altered mRNA level in nontumorigenic Wilms' microcell hybrid cells.

Publication ,  Journal Article
Dowdy, SF; Lai, KM; Weissman, BE; Matsui, Y; Hogan, BL; Stanbridge, EJ
Published in: Nucleic Acids Res
October 25, 1991

Wilms' tumor, a pediatric nephroblastoma, has been associated with genetic alterations of the 11p13 and 11p15 regions. The introduction of a der(11) chromosome into the G401 Wilms' tumor cell line has been shown previously to revert the tumorigenic phenotype. A subtractive cDNA/RNA hybridization performed between the tumorigenic parent (G401) and a nontumorigenic microcell hybrid of G401 (110.1/G401.1) containing the der(11) chromosome resulted in the identification of a single novel cDNA clone, designated QM. The cDNA is 745 nucleotides in length and encodes a predicted hydrophilic 25 kd basic protein, primarily consisting of alpha helices. The QM transcript is expressed in a wide variety of embryonic and adult tissues and demonstrates a down regulation of expression in adult kidney and heart. QM is also a member of a multigene family members of which map to chromosomes 6 and 14. The QM mRNA level is modulated between the tumorigenic and nontumorigenic cell lines and therefore may be involved in the maintenance of the nontumorigenic phenotype.

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Published In

Nucleic Acids Res

DOI

ISSN

0305-1048

Publication Date

October 25, 1991

Volume

19

Issue

20

Start / End Page

5763 / 5769

Location

England

Related Subject Headings

  • Wilms Tumor
  • Tumor Cells, Cultured
  • RNA, Messenger
  • Organ Specificity
  • Nucleic Acid Hybridization
  • Molecular Sequence Data
  • Hybrid Cells
  • Humans
  • Genes, Tumor Suppressor
  • Developmental Biology
 

Citation

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Dowdy, S. F., Lai, K. M., Weissman, B. E., Matsui, Y., Hogan, B. L., & Stanbridge, E. J. (1991). The isolation and characterization of a novel cDNA demonstrating an altered mRNA level in nontumorigenic Wilms' microcell hybrid cells. Nucleic Acids Res, 19(20), 5763–5769. https://doi.org/10.1093/nar/19.20.5763
Dowdy, S. F., K. M. Lai, B. E. Weissman, Y. Matsui, B. L. Hogan, and E. J. Stanbridge. “The isolation and characterization of a novel cDNA demonstrating an altered mRNA level in nontumorigenic Wilms' microcell hybrid cells.Nucleic Acids Res 19, no. 20 (October 25, 1991): 5763–69. https://doi.org/10.1093/nar/19.20.5763.
Dowdy SF, Lai KM, Weissman BE, Matsui Y, Hogan BL, Stanbridge EJ. The isolation and characterization of a novel cDNA demonstrating an altered mRNA level in nontumorigenic Wilms' microcell hybrid cells. Nucleic Acids Res. 1991 Oct 25;19(20):5763–9.
Dowdy, S. F., et al. “The isolation and characterization of a novel cDNA demonstrating an altered mRNA level in nontumorigenic Wilms' microcell hybrid cells.Nucleic Acids Res, vol. 19, no. 20, Oct. 1991, pp. 5763–69. Pubmed, doi:10.1093/nar/19.20.5763.
Dowdy SF, Lai KM, Weissman BE, Matsui Y, Hogan BL, Stanbridge EJ. The isolation and characterization of a novel cDNA demonstrating an altered mRNA level in nontumorigenic Wilms' microcell hybrid cells. Nucleic Acids Res. 1991 Oct 25;19(20):5763–5769.
Journal cover image

Published In

Nucleic Acids Res

DOI

ISSN

0305-1048

Publication Date

October 25, 1991

Volume

19

Issue

20

Start / End Page

5763 / 5769

Location

England

Related Subject Headings

  • Wilms Tumor
  • Tumor Cells, Cultured
  • RNA, Messenger
  • Organ Specificity
  • Nucleic Acid Hybridization
  • Molecular Sequence Data
  • Hybrid Cells
  • Humans
  • Genes, Tumor Suppressor
  • Developmental Biology