A mouse homologue of FAST-1 transduces TGF beta superfamily signals and is expressed during early embryogenesis.

Published

Journal Article

The transcription factor FAST-1 has recently been shown to play a key role in the specification of mesoderm by TGF beta superfamily signals in the early Xenopus embryo. We have cloned Fast1, a mouse homologue of Xenopus FAST-1, and characterized its expression during embryogenesis and function in activin/TGF beta signal transduction. In vitro, Fast1 associates with Smads in response to an activin/TGF beta signal to form a complex that recognizes the Xenopus activin responsive element (ARE) targeted by Xenopus FAST-1. In intact cells, introduction of Fast1 confers activin/TGF beta regulation of an ARE-luciferase reporter. In embryos, Fast1 is expressed predominantly throughout the epiblast before gastrulation and declines as development progresses. We propose that mouse Fast1, like Xenopus FAST-1, mediates TGF beta superfamily signals specifying developmental fate during early embryogenesis.

Full Text

Duke Authors

Cited Authors

  • Weisberg, E; Winnier, GE; Chen, X; Farnsworth, CL; Hogan, BL; Whitman, M

Published Date

  • December 1998

Published In

Volume / Issue

  • 79 / 1-2

Start / End Page

  • 17 - 27

PubMed ID

  • 10349617

Pubmed Central ID

  • 10349617

Electronic International Standard Serial Number (EISSN)

  • 1872-6356

International Standard Serial Number (ISSN)

  • 0925-4773

Digital Object Identifier (DOI)

  • 10.1016/s0925-4773(98)00160-9

Language

  • eng