Molecular and phenotypic characterization of a new mouse insertional mutation that causes a defect in the distal vertebrae of the spine.

Journal Article

We have identified and characterized the phenotype of a new insertional mutation in one line of transgenic mice. Mice carrying this mutation, which we have designated TgN(Imusd)370Rpw, display undulations of the vertebrae giving rise to a novel kinky-tail phenotype. Molecular characterization of the insertion site indicates that the transgene integration has occurred without any substantial alterations in the structure of the host sequences. Using probes that flank the insertion site, we have mapped the mutation to chromosome 5 near the semidominant mutation, thick tail (Tht). Thick tail does not complement the TgN(Imusd)370Rpw mutation; compound mutants containing one copy of each mutation display a more severe phenotype than either mutation individually.

Full Text

Duke Authors

Cited Authors

  • Schrick, JJ; Dickinson, ME; Hogan, BL; Selby, PB; Woychik, RP

Published Date

  • July 1995

Published In

Volume / Issue

  • 140 / 3

Start / End Page

  • 1061 - 1067

PubMed ID

  • 7672577

International Standard Serial Number (ISSN)

  • 0016-6731

Language

  • eng

Conference Location

  • United States