In vitro synthesis of type IV procollagen.
Journal Article (Journal Article)
Total RNA was isolated from parietal endoderm cells of 131/2-day mouse embryos that synthesize large amounts of type IV procollagen. In vitro translation of this RNA in the reticulocyte lysate supplemented with a ribonuclease inhibitor yielded two equally prominent polypeptides of Mr = 165,000 and 168,000, immunoprecipitable with anti-mouse type IV collagen serum. The Mr = 165,000 polypeptide was shown by one-dimensional peptide mapping to represent an unmodified chain of type IV procollagen. The Mr = 168,000 polypeptide, the in vitro synthesis of which was barely detectable in the absence of a ribonuclease inhibitor, most likely represents the other genetically distinct chain of type IV procollagen. Similar results to those described were also obtained using poly(A) + RNA prepared from murine F9 embryonal carcinoma cells induced to differentiate in vitro into parietal endoderm.
Full Text
Duke Authors
Cited Authors
- Kurkinen, M; Foster, L; Barlow, DP; Hogan, BL
Published Date
- December 25, 1982
Published In
Volume / Issue
- 257 / 24
Start / End Page
- 15151 - 15155
PubMed ID
- 6184371
International Standard Serial Number (ISSN)
- 0021-9258
Language
- eng
Conference Location
- United States