In vitro synthesis of type IV procollagen.

Journal Article

Total RNA was isolated from parietal endoderm cells of 131/2-day mouse embryos that synthesize large amounts of type IV procollagen. In vitro translation of this RNA in the reticulocyte lysate supplemented with a ribonuclease inhibitor yielded two equally prominent polypeptides of Mr = 165,000 and 168,000, immunoprecipitable with anti-mouse type IV collagen serum. The Mr = 165,000 polypeptide was shown by one-dimensional peptide mapping to represent an unmodified chain of type IV procollagen. The Mr = 168,000 polypeptide, the in vitro synthesis of which was barely detectable in the absence of a ribonuclease inhibitor, most likely represents the other genetically distinct chain of type IV procollagen. Similar results to those described were also obtained using poly(A) + RNA prepared from murine F9 embryonal carcinoma cells induced to differentiate in vitro into parietal endoderm.

Full Text

Duke Authors

Cited Authors

  • Kurkinen, M; Foster, L; Barlow, DP; Hogan, BL

Published Date

  • December 25, 1982

Published In

Volume / Issue

  • 257 / 24

Start / End Page

  • 15151 - 15155

PubMed ID

  • 6184371

International Standard Serial Number (ISSN)

  • 0021-9258

Language

  • eng

Conference Location

  • United States