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Structure of the crystalline bilayer in the subgel phase of dipalmitoylphosphatidylglycerol.

Publication ,  Journal Article
Blaurock, AE; McIntosh, TJ
Published in: Biochemistry
January 28, 1986

The structure of the subgel phase of dipalmitoylphosphatidylglycerol (DPPG) has been analyzed by X-ray diffraction techniques. Diffraction recorded from highly oriented DPPG specimens in the subgel phase extends to 2-A resolution. There are sharp lamellar reflections on the meridian, and other reflections lie on a series of wide-angle lattice lines parallel to the meridian and crossing the equator in the range of 8-2 A. The wide-angle lattice lines consist of radially sharp reflections centered on the equator of the X-ray film and also a series of broader, off-equatorial maxima. The lattice lines indicate that the DPPG molecules in each bilayer crystallize in a two-dimensional oblique lattice with dimensions a = 5.50 A, b = 7.96 A, and gamma = 100.5 degrees. These oblique lattices are not regularly aligned from bilayer to bilayer. Analysis of the lamellar diffraction shows that the bilayer has about the same thickness in the subgel and gel (L beta') phases. In the direction normal to the hydrocarbon chains, the chains are significantly closer together in the subgel phase as compared to the normal L beta' gel phase but have about the same separation as the chains in polyethylene and the crystalline n-alkanes. The bilayer thickness, area per lipid molecule, and intensity distribution along the lattice lines all indicate that in the subgel phase the hydrocarbon chains are tilted between 30 and 35 degrees from the normal to the bilayer plane.(ABSTRACT TRUNCATED AT 250 WORDS)

Duke Scholars

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

January 28, 1986

Volume

25

Issue

2

Start / End Page

299 / 305

Location

United States

Related Subject Headings

  • X-Ray Diffraction
  • Phosphatidylglycerols
  • Molecular Conformation
  • Models, Molecular
  • Lipid Bilayers
  • Gels
  • Crystallization
  • Biochemistry & Molecular Biology
  • 3404 Medicinal and biomolecular chemistry
  • 3205 Medical biochemistry and metabolomics
 

Citation

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Blaurock, A. E., & McIntosh, T. J. (1986). Structure of the crystalline bilayer in the subgel phase of dipalmitoylphosphatidylglycerol. Biochemistry, 25(2), 299–305. https://doi.org/10.1021/bi00350a003
Blaurock, A. E., and T. J. McIntosh. “Structure of the crystalline bilayer in the subgel phase of dipalmitoylphosphatidylglycerol.Biochemistry 25, no. 2 (January 28, 1986): 299–305. https://doi.org/10.1021/bi00350a003.
Blaurock AE, McIntosh TJ. Structure of the crystalline bilayer in the subgel phase of dipalmitoylphosphatidylglycerol. Biochemistry. 1986 Jan 28;25(2):299–305.
Blaurock, A. E., and T. J. McIntosh. “Structure of the crystalline bilayer in the subgel phase of dipalmitoylphosphatidylglycerol.Biochemistry, vol. 25, no. 2, Jan. 1986, pp. 299–305. Pubmed, doi:10.1021/bi00350a003.
Blaurock AE, McIntosh TJ. Structure of the crystalline bilayer in the subgel phase of dipalmitoylphosphatidylglycerol. Biochemistry. 1986 Jan 28;25(2):299–305.
Journal cover image

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

January 28, 1986

Volume

25

Issue

2

Start / End Page

299 / 305

Location

United States

Related Subject Headings

  • X-Ray Diffraction
  • Phosphatidylglycerols
  • Molecular Conformation
  • Models, Molecular
  • Lipid Bilayers
  • Gels
  • Crystallization
  • Biochemistry & Molecular Biology
  • 3404 Medicinal and biomolecular chemistry
  • 3205 Medical biochemistry and metabolomics