The extracellular nuclease of Staphylococcus aureus: structures of the native enzyme and an enzyme-inhibitor complex at 4 A resolution.

Journal Article (Journal Article)

Independent 4 A electron density maps calculated for the extracellular nuclease of Staphylococcus aureus (based on data from three heavy-atom derivatives) and for a nuclease-thymidine-3',5'-diphosphate-calcium ion complex (based on a single isomorphous derivative) show about 60 per cent of the chain resolved, including 3(1/2) turns of helix. The pyrimidine ring of the inhibitor fits into a pocket in the enzyme and appears to be parallel to the ring of a tyrosyl residue. Conformational changes can be observed between the nuclease and the nuclease-inhibitor complex, but the two structures seem to be identical over most of the molecule.

Full Text

Duke Authors

Cited Authors

  • Arnone, A; Bier, CJ; Cotton, FA; Hazen, EE; Richardson, DC; Richardson, JS

Published Date

  • October 1, 1969

Published In

Volume / Issue

  • 64 / 2

Start / End Page

  • 420 - 427

PubMed ID

  • 5261023

Pubmed Central ID

  • PMC223359

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.64.2.420


  • eng

Conference Location

  • United States