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The extracellular nuclease of Staphylococcus aureus: structures of the native enzyme and an enzyme-inhibitor complex at 4 A resolution.

Publication ,  Journal Article
Arnone, A; Bier, CJ; Cotton, FA; Hazen, EE; Richardson, DC; Richardson, JS
Published in: Proc Natl Acad Sci U S A
October 1969

Independent 4 A electron density maps calculated for the extracellular nuclease of Staphylococcus aureus (based on data from three heavy-atom derivatives) and for a nuclease-thymidine-3',5'-diphosphate-calcium ion complex (based on a single isomorphous derivative) show about 60 per cent of the chain resolved, including 3(1/2) turns of helix. The pyrimidine ring of the inhibitor fits into a pocket in the enzyme and appears to be parallel to the ring of a tyrosyl residue. Conformational changes can be observed between the nuclease and the nuclease-inhibitor complex, but the two structures seem to be identical over most of the molecule.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 1969

Volume

64

Issue

2

Start / End Page

420 / 427

Location

United States

Related Subject Headings

  • X-Ray Diffraction
  • Staphylococcus
  • Ribonucleases
  • Nucleotidyltransferases
  • Molecular Weight
  • Models, Chemical
  • Deoxyribonucleases
  • Crystallization
 

Citation

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Arnone, A., Bier, C. J., Cotton, F. A., Hazen, E. E., Richardson, D. C., & Richardson, J. S. (1969). The extracellular nuclease of Staphylococcus aureus: structures of the native enzyme and an enzyme-inhibitor complex at 4 A resolution. Proc Natl Acad Sci U S A, 64(2), 420–427. https://doi.org/10.1073/pnas.64.2.420
Arnone, A., C. J. Bier, F. A. Cotton, E. E. Hazen, D. C. Richardson, and J. S. Richardson. “The extracellular nuclease of Staphylococcus aureus: structures of the native enzyme and an enzyme-inhibitor complex at 4 A resolution.Proc Natl Acad Sci U S A 64, no. 2 (October 1969): 420–27. https://doi.org/10.1073/pnas.64.2.420.
Arnone A, Bier CJ, Cotton FA, Hazen EE, Richardson DC, Richardson JS. The extracellular nuclease of Staphylococcus aureus: structures of the native enzyme and an enzyme-inhibitor complex at 4 A resolution. Proc Natl Acad Sci U S A. 1969 Oct;64(2):420–7.
Arnone, A., et al. “The extracellular nuclease of Staphylococcus aureus: structures of the native enzyme and an enzyme-inhibitor complex at 4 A resolution.Proc Natl Acad Sci U S A, vol. 64, no. 2, Oct. 1969, pp. 420–27. Pubmed, doi:10.1073/pnas.64.2.420.
Arnone A, Bier CJ, Cotton FA, Hazen EE, Richardson DC, Richardson JS. The extracellular nuclease of Staphylococcus aureus: structures of the native enzyme and an enzyme-inhibitor complex at 4 A resolution. Proc Natl Acad Sci U S A. 1969 Oct;64(2):420–427.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 1969

Volume

64

Issue

2

Start / End Page

420 / 427

Location

United States

Related Subject Headings

  • X-Ray Diffraction
  • Staphylococcus
  • Ribonucleases
  • Nucleotidyltransferases
  • Molecular Weight
  • Models, Chemical
  • Deoxyribonucleases
  • Crystallization