In vivo transfer of GPI-linked complement restriction factors from erythrocytes to the endothelium.

Published

Journal Article

Many proteins are associated with the outer layer of the cell membrane through a posttranslationally added glycosyl phosphatidylinositol (GPI) anchor. The functional significance of this type of protein linkage is unclear, although it results in increased lateral mobility, sorting to the apical surface of the cell, reinsertion into cell membranes, and possibly cell signaling. Here evidence is presented that GPI-linked proteins can undergo intermembrane transfer in vivo. GPI-linked proteins expressed on the surface of transgenic mouse red blood cells were transferred in a functional form to endothelial cells in vivo. This feature of GPI linkage may be potentially useful for the delivery of therapeutic proteins to vascular endothelium.

Full Text

Duke Authors

Cited Authors

  • Kooyman, DL; Byrne, GW; McClellan, S; Nielsen, D; Tone, M; Waldmann, H; Coffman, TM; McCurry, KR; Platt, JL; Logan, JS

Published Date

  • July 7, 1995

Published In

Volume / Issue

  • 269 / 5220

Start / End Page

  • 89 - 92

PubMed ID

  • 7541557

Pubmed Central ID

  • 7541557

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.7541557

Language

  • eng

Conference Location

  • United States