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Neuroendocrine effects of dehydration in mice lacking the angiotensin AT1a receptor.

Publication ,  Journal Article
Morris, M; Li, P; Callahan, MF; Oliverio, MI; Coffman, TM; Bosch, SM; Diz, DI
Published in: Hypertension
January 1999

Angiotensin (Ang) type 1a (AT1a) receptors are critical in the control of blood pressure and water balance. Experiments were performed to determine the influence of dehydration on brain Ang receptors and plasma vasopressin (VP) in mice lacking this receptor. Control or AT1a knockout (AT1aKO) male mice were give water ad libitum or deprived of water for 48 hours. Animals were anesthetized with halothane, blood samples were collected by heart puncture, and brains were processed for Ang-receptor autoradiography with 125I-sarthran (0.4 nmol/L). Dehydration produced an increase in AT1 receptors in the paraventricular nucleus (PVN) and anterior pituitary (AP) in control mice (PVN: 70+/-16 versus 146+/-10 fmol/mg protein; AP: 41+/-7 versus 86+/-15 fmol/mg protein). No changes were noted in the median preoptic nucleus. The majority of the brain receptors were of the AT1 subtype. There was little or no specific Ang binding in AT1aKO mice and no effect of dehydration. Plasma VP levels were elevated in the halothane-anesthetized animals (>200 pg/mL) with no significant effect of dehydration. A separate experiment was performed with decapitated mice anesthetized with pentobarbital. Dehydration increased plasma VP in control mice, from 3.3+/-0.6 to 13.3+/-4.7 pg/mL, whereas no change was noted in the AT1aKO mice, 5.1+/-0.3 versus 6.1+/-0.7 pg/mL (water versus dehydration). These results demonstrate a differential response to dehydration in mice lacking AT1a receptors. There was no evidence for AT1 receptors of any subtype in the brain regions examined and no effect of dehydration on VP secretion or brain Ang receptors.

Duke Scholars

Published In

Hypertension

ISSN

0194-911X

Publication Date

January 1999

Volume

33

Issue

1 Pt 2

Start / End Page

482 / 486

Location

united states

Related Subject Headings

  • Cardiovascular System & Hematology
  • 1117 Public Health and Health Services
  • 1103 Clinical Sciences
  • 1102 Cardiorespiratory Medicine and Haematology
 

Citation

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Morris, M., Li, P., Callahan, M. F., Oliverio, M. I., Coffman, T. M., Bosch, S. M., & Diz, D. I. (1999). Neuroendocrine effects of dehydration in mice lacking the angiotensin AT1a receptor. Hypertension, 33(1 Pt 2), 482–486.
Morris, M., P. Li, M. F. Callahan, M. I. Oliverio, T. M. Coffman, S. M. Bosch, and D. I. Diz. “Neuroendocrine effects of dehydration in mice lacking the angiotensin AT1a receptor.Hypertension 33, no. 1 Pt 2 (January 1999): 482–86.
Morris M, Li P, Callahan MF, Oliverio MI, Coffman TM, Bosch SM, et al. Neuroendocrine effects of dehydration in mice lacking the angiotensin AT1a receptor. Hypertension. 1999 Jan;33(1 Pt 2):482–6.
Morris, M., et al. “Neuroendocrine effects of dehydration in mice lacking the angiotensin AT1a receptor.Hypertension, vol. 33, no. 1 Pt 2, Jan. 1999, pp. 482–86.
Morris M, Li P, Callahan MF, Oliverio MI, Coffman TM, Bosch SM, Diz DI. Neuroendocrine effects of dehydration in mice lacking the angiotensin AT1a receptor. Hypertension. 1999 Jan;33(1 Pt 2):482–486.

Published In

Hypertension

ISSN

0194-911X

Publication Date

January 1999

Volume

33

Issue

1 Pt 2

Start / End Page

482 / 486

Location

united states

Related Subject Headings

  • Cardiovascular System & Hematology
  • 1117 Public Health and Health Services
  • 1103 Clinical Sciences
  • 1102 Cardiorespiratory Medicine and Haematology