An Escherichia coli mutant defective in lipid export.

Published

Journal Article

Escherichia coli phospholipids and lipopolysaccharide, made on the inner surface of the inner membrane, are rapidly transported to the outer membrane by mechanisms that are not well characterized. We now report a temperature-sensitive mutant (WD2) with an A270T substitution in a trans-membrane region of the ABC transporter MsbA. As shown by (32)P(i) and (14)C-acetate labeling, export of all major lipids to the outer membrane is inhibited by approximately 90% in WD2 after 30 min at 44 degrees C. Transport of newly synthesized proteins is not impaired. Electron microscopy shows reduplicated inner membranes in WD2 at 44 degrees C, consistent with a key role for MsbA in lipid trafficking.

Full Text

Duke Authors

Cited Authors

  • Doerrler, WT; Reedy, MC; Raetz, CR

Published Date

  • April 2001

Published In

Volume / Issue

  • 276 / 15

Start / End Page

  • 11461 - 11464

PubMed ID

  • 11278265

Pubmed Central ID

  • 11278265

Electronic International Standard Serial Number (EISSN)

  • 1083-351X

International Standard Serial Number (ISSN)

  • 0021-9258

Digital Object Identifier (DOI)

  • 10.1074/jbc.c100091200

Language

  • eng