Coordinate changes in C protein and myosin expression during skeletal muscle hypertrophy.

Published

Journal Article

In this study, two new C protein isoforms in adult rat skeletal muscle were resolved using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These isoforms migrated between previously identified fast (Cf) and slow (Cs) C protein isoforms; hence they were named intermediate C proteins (Ci1 and Ci2). Cyanogen bromide peptide mapping and Western blotting showed that the intermediate isoforms were more similar to Cs than Cf. The distribution of specific C protein and myosin heavy chain (MHC) isoforms was highly correlated in several hindlimb muscles, suggesting that the expression of these two thick-filament proteins is coordinated. This notion was tested by determining whether specific C protein and MHC isoforms change in parallel during muscle hypertrophy. Eight weeks after ablation of its synergists, the overloaded plantaris muscle showed significant increases in type IIa MHC and intermediate C protein, with corresponding decreases in type IIb MHC and Cf protein. These results indicate that C protein expression is linked to MHC expression during plantaris muscle hypertrophy.

Full Text

Duke Authors

Cited Authors

  • McCormick, KM; Baldwin, KM; Schachat, F

Published Date

  • August 1994

Published In

Volume / Issue

  • 267 / 2 Pt 1

Start / End Page

  • C443 - C449

PubMed ID

  • 8074179

Pubmed Central ID

  • 8074179

International Standard Serial Number (ISSN)

  • 0002-9513

Digital Object Identifier (DOI)

  • 10.1152/ajpcell.1994.267.2.C443

Language

  • eng

Conference Location

  • United States