The suppression of testis-brain RNA binding protein and kinesin heavy chain disrupts mRNA sorting in dendrites.

Published

Journal Article

Ribonucleoprotein particles (RNPs) are thought to be key players in somato-dendritic sorting of mRNAs in CNS neurons and are implicated in activity-directed neuronal remodeling. Here, we use reporter constructs and gel mobility shift assays to show that the testis brain RNA-binding protein (TB-RBP) associates with mRNPs in a sequence (Y element) dependent manner. Using antisense oligonucleotides (anti-ODN), we demonstrate that blocking the TB-RBP Y element binding site disrupts and mis-localizes mRNPs containing (alpha)-calmodulin dependent kinase II (alpha)-CAMKII) and ligatin mRNAs. In addition, we show that suppression of kinesin heavy chain motor protein alters only the localization of (alpha)-CAMKII mRNA. Thus, differential sorting of mRNAs involves multiple mRNPs and selective motor proteins permitting localized mRNAs to utilize common mechanisms for shared steps.

Full Text

Duke Authors

Cited Authors

  • Severt, WL; Biber, TU; Wu, X; Hecht, NB; DeLorenzo, RJ; Jakoi, ER

Published Date

  • November 1999

Published In

Volume / Issue

  • 112 ( Pt 21) /

Start / End Page

  • 3691 - 3702

PubMed ID

  • 10523505

Pubmed Central ID

  • 10523505

International Standard Serial Number (ISSN)

  • 0021-9533

Language

  • eng

Conference Location

  • England