Immunoreactive forms of human erythrocyte ankyrin are present in diverse cells and tissues.

Journal Article

Ankyrin is a polypeptide of molecular weight (MW) 200,000 which is tightly bound to the cytoplasmic surface of the human erythrocyte membrane and has been identified as the high-affinity membrane attachment protein for spectrin. This protein has also been shown to be associated with band 3 (ref. 4), the major transmembrane protein which links a cytoplasmic structural protein to an integral membrane protein. A water-soluble, 72,000-MW, proteolytic fragment of ankyrin has been purified which retains the ability to bind to spectrin, and competitively inhibits reassociation of spectrin with membranes. Monospecific antibodies directed against this fragment have been prepared and demonstrated to cross-react only with ankyrin among the erythrocyte membrane proteins. The present study reports the use of these antibodies to develop a radioimmunoassay capable of detecting femtomolar quantities of ankyrin, and demonstrates the presence of small but significant amounts of immunoreactivity in a variety of types of cells and tissues.

Full Text

Duke Authors

Cited Authors

  • Bennett, V

Published Date

  • October 18, 1979

Published In

Volume / Issue

  • 281 / 5732

Start / End Page

  • 597 - 599

PubMed ID

  • 492324

International Standard Serial Number (ISSN)

  • 0028-0836

Language

  • eng

Conference Location

  • England