Colocalization of band 3 with ankyrin and spectrin at the basal membrane of intercalated cells in the rat kidney.
Journal Article (Journal Article)
An immunoreactive form of the anion channel protein of erythrocytes, band 3, has been identified in the rat kidney. It is found in the intercalated cells of the distal tubule and collecting ducts. Immunostaining specific for band 3 is confined to the basolateral plasma membrane of these cells, where this protein probably mediates the transport of bicarbonate across the tubular wall. Double-immunolabeling studies demonstrate that band 3 is colocalized with immunoreactive forms of ankyrin and spectrin along the basolateral plasma membrane. The polarized distribution of band 3 may be the result of the association of its cytoplasmic domain with ankyrin, which in turn links band 3 to spectrin and the cytoskeleton. These observations help to explain how the collecting ducts of the kidney can direct the transport of bicarbonate ions, thus maintaining the acid-base balance.
Full Text
Duke Authors
Cited Authors
- Drenckhahn, D; Schlüter, K; Allen, DP; Bennett, V
Published Date
- December 13, 1985
Published In
Volume / Issue
- 230 / 4731
Start / End Page
- 1287 - 1289
PubMed ID
- 2933809
International Standard Serial Number (ISSN)
- 0036-8075
Digital Object Identifier (DOI)
- 10.1126/science.2933809
Language
- eng
Conference Location
- United States