Fibronectin isoforms in megakaryocytes.

Published

Journal Article

Our studies have shown that megakaryocytes (MK) can synthesize fibronectin (FN) and alternatively spliced fibronectin, FN EIIIB. FN EIIIB is primarily present in embryonic, proliferating and migrating cells, and thought to be important for cell maturation. MK, but not nonmegakaryocytic bone marrow cells, contain FN EIIIB and thus, MK and platelets are among a small number of adult cells and tissues that synthesize and contain FN EIIIB. Thrombin can induce the secretion of general FN, but does not cause the secretion of FN EIIIB into the medium. Analysis of immunostained cells by confocal microscopy revealed that both general FN and FN EIIIB accumulated on the MK surface following thrombin treatment. Thus, FN EIIIB can be released only to be bound to the MK surface. The expression of FN EIIIB on the MK surface may have a unique role in MK migration and maturation.

Full Text

Duke Authors

Cited Authors

  • Schick, PK; Wojensk, CM; Bennett, V; Denisova, L

Published Date

  • 1996

Published In

Volume / Issue

  • 14 Suppl 1 /

Start / End Page

  • 212 - 219

PubMed ID

  • 11012224

Pubmed Central ID

  • 11012224

International Standard Serial Number (ISSN)

  • 1066-5099

Digital Object Identifier (DOI)

  • 10.1002/stem.5530140728

Language

  • eng

Conference Location

  • United States