Dynamic actin structures stabilized by profilin.


Journal Article

We describe the production and analysis of clonal cell lines in which we have overexpressed human profilin, a small ubiquitous actin monomer binding protein, to assess the role of profilin on actin function in vivo. The concentration of filamentous actin is increased in cells with higher profilin levels, and actin filament half-life measured in these cells is directly proportional to the steady-state profilin concentration. The distribution of actin filaments is altered by profilin overexpression. While parallel actin bundles crossing the cells are virtually absent in cells overexpressing profilin, the submembranous actin network of these cells is denser than in control cells. These results suggest that in vivo profilin regulates the stability, and thereby distribution, of specific dynamic actin structures.

Full Text

Cited Authors

  • Finkel, T; Theriot, JA; Dise, KR; Tomaselli, GF; Goldschmidt-Clermont, PJ

Published Date

  • February 1994

Published In

Volume / Issue

  • 91 / 4

Start / End Page

  • 1510 - 1514

PubMed ID

  • 8108438

Pubmed Central ID

  • 8108438

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.91.4.1510


  • eng